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Course Detail

Course Name Pharmaceutical Microbiology Practical
Course Code PD.209P
Program Pharm. D.
Campus Kochi

Syllabus

LIST OF EXPERIMENTS:

  1. Study of apparatus used in experimental
  2. Sterilization of glass
  3. Preparation of culture media for bacteria and
  4. Staining techniques using different microbial samples –
    1. Simple staining
    2. Gram’s staining
    3. Negative staining
    4. Acid fast
  5. Study of motility characters of
  6. Enumeration of microorganisms –
    1. Total count techniques
    2. Viable count
  7. Study of different methods of isolation of pure cultures –
    1. Spread plate technique
    2. Pour plate technique
    3. Streak plate techniques – simple streaking & multiple streaking
  8. Biochemical testing for the identification of
  9. Culture sensitivity testing for some
  10. Sterility testing for sterile dosage
  11. Determination of minimum inhibitory
  12. Microbiological assay of antibiotics by cup plate

 

 

SEMINAR/ASSIGNMENTS:

 

 

  1. Review the significance of microbiology in pharmaceutical sciences by highlighting key contributors and major breakthroughs in drug development, vaccines, and infection
  2. Explain the mechanisms of multi-drug resistance (MDR) and discuss its pharmaceutical challenges with relevant clinical examples.
  3. Describe the structure, pathophysiology, and clinical impact of SARS-CoV-2 and its major variants, with emphasis on pharmaceutical relevance.
  4. Evaluate the application of Omics technologies (Genomics, Proteomics, Metabolomics) in studying microbial metabolites and their role in pharmaceutical drug discovery.
  5. Prepare a report on the current status of antibiotic resistance, assess surveillance methods, and propose strategies to combat antimicrobial resistance.

 

  1. Discuss recent microbial diagnostic techniques, focusing on emerging infectious diseases and highlighting tools like PCR, CRISPR diagnostics, and biosensors.

Text Books / References

TEXT BOOKS:

 

  1. Chandrakant       Kokare.      Pharmaceutical       Microbiology.       9th      edition, NiraliPrakashan,Pune, 2022.
  2. Pharmaceutical Microbiology.1st edition, New Age International Ltd Publishers,New Delhi, 2019.
  3. Ananthanarayan and Panicker’s.Text book of 12th edition,Orient Longman Ltd, Chennai,2022.
  4. Prescott and Industrial Microbiology. 4th edition. CBS Publishers & Distributors, New Delhi, 2005.

 

REFERENCE BOOKS

 

  1. Pelczar, Chan Kreig.Microbiology. 5th edition, Tata McGraw Hill edition, NewDelhi,
  2. Prescot M, Jarley G.P, Klein D.A. Microbiology. 2nd edition, Mc Graw Hill Company Inc., 2005.
  3. E Bennett , R. Dolin R (Eds): Principles and Practice of Infectious Disease. 4th edition. Churchill Livingstone, New York, 2002.
  4. .Seth K. Pharmaceutical Microbiology (A Laboratory Hand Book).1st edition, S. Vikas & Co. Publishing House, Jalandhar , 2018.

 

 

*Latest edition of the text books & reference books can be referred


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