• Gating strategies for immune cell identification (T cells, B cells, and NK cells)
• Fluorescent compensation and spillover correction.
• Bigfoot software tools for data visualization and statistical analysis Analysis of disease-specific immune phenotypes

• Cell sorting principles and instrument setup
• Purity and recovery optimization
• Downstream applications (RNA extraction, functional assays, culture)
• Safety protocols and ethical considerations

Participant Division:

Participants will be divided into two groups (A & B) and will rotate between lab stations on both days to ensure equal exposure and comprehensive learning

Target Participants:

• Early-career researchers, clinicians, and PhD scholars in biomedical sciences.
• Translational research teams from academia and industry.

Expected Outcomes:

• Basic Understanding of Immune Cell Profiling and Sorting
• Translational Research Competency in Immunology
• Hands-On Technical Proficiency and Analytical Skills

Support requirements:

• Logistical support for travel and accommodation of invited faculty
• Funding for workshop materials, antibodies, and reagents 
• Technical support from ThermoFisher Scientific for instrument and software assistance
• Promotion through academic networks and institutional platforms
• Administrative support for participant registration and documentation