Day 1: Monday April 7, 2025
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Time
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Program
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8:30 AM-9.00AM
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Registration
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9:00AM- 9:30 AM
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Inauguration, Overview of workshop and Introduction of participants
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Theme1:Know Your Flow Cytometer
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9:30AM- 10:00AM
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Expert Talk on “The Importance of Flow Cytometry in Biomedical Research and Diagnostics”
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10.00 AM – 11.00 AM
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Understanding the technology of Spectral Flowcytometry and its difference from Conventional Flowcytometry
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11:00AM – 12:00PM
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Basics of Flowcytometry and cell sorting and its components (principles and instrumentation)
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12:00PM – 1:30PM
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Practical Demonstration of BigFoot Cell sorter:Components and Operation (including routine checks ofperformance)
- Basic Instrument set-up,
- Quality Check
- Cleaningcycles of Cell Sorter cum Flow analyzer
- Understanding basics of Drop delay
- Nozzles
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1:30 PM – 2:30 PM
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Lunch
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Theme 2: Applications of Flowcytometer and cell sorter
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2:30 PM – 3:00 PM
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Application of Flowcytometer: Cell Cycle; Cell viability; Apoptosis; Immunophenotyping and Applications of Cell sorting
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3:00 PM-3:30 PM
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Application in Detail: Cell Analysis using PI and Thermo Fisher Vibrant dye cycle
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3:30 PM-5:30 PM
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Wet Lab: Cell Cycle: Cell Preparation and Staining
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3:30 PM – 4:30 PM
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Batch 1: Cell Cycle sample run on BigFoot
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4:30 PM – 5:30 PM
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Batch 2: Cell Cycle sample run on BigFoot
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5:30 PM – 6:00 PM
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Q & A session
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Day 2: Tuesday April 8, 2025
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Time
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Program
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9:00AM- 10:00 AM
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Spectral Flowcytometry and Panel Design
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10:00 AM – 10:30 AM
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Understanding Basic Immunophenotyping: Immune Cell Population and their markers
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10:30 AM – 11:30 AM
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Application in Detail: Immunophenotyping – Surface and Intracellular: Choice of right fluoro chromes and antibodies, Spectral overlap, Voltage settings, Importance of experiment, data acquisition andcontrols.
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11:30 AM – 1:30PM
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Wet Lab: Hands-on training in Peripheral Blood Mononuclear Cell Isolation and Discussion
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1:30 PM – 2:30 PM
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Lunch
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Batch 1
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Batch 2
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3:30 PM – 4:30 PM
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Wet Lab: Surface Antibody staining of cells
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Acquisition in BigFoot and Analysis
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Acquisition in BigFoot and Analysis Spectral overlap,
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Wet Lab: Surface Antibody staining of cells
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4:30 PM – 5:30 PM
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Data Analysis: Cell Cycle and Immunophenotyping
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5:30 PM – 6:30 PM
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Quiz and Valedictory
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