Day 1: Foundations & Practical Applications In Immune Cell Isolation
| Time |
Programme |
| 08:30 AM – 09:30 AM |
Registration |
| Session 1 – Inauguration |
| 09:30 AM – 09:35 AM |
Pooja Ceremony |
| 09:35 AM – 09:50 AM |
Welcome Address and Introduction of Participants |
| 09:50 AM – 10:00 AM |
Overview of Symposium Objectives |
| 10:00 AM – 10:30 AM |
Keynote: From Immune Discovery to Diagnostic Innovation – Role of Advanced Flow Cytometry |
| 10:30 AM – 10:45 AM |
Tea Break |
| Session 2 – Flow Cytometry in Translational Immunology |
| 10:45 AM – 11:15 AM |
Immunophenotyping with Bigfoot Flow Cytometer: Multi-Parameter Panel Design |
| 11:15 AM – 11:45 AM |
Advanced Immune Cell Isolation Techniques: Principles and Best Practices |
| 11:45 AM – 12:15 PM |
Flow Cytometry in Clinical Diagnostics: Disease Monitoring and Biomarker Discovery |
| 12:15 PM – 01:00 PM |
Panel Discussion |
| 01:00 PM – 02:00 PM |
Lunch |
| Lab Session 1 (Groups A & B Rotate) |
| 02:00 PM – 03:30 PM |
Lab 1A (Group A): Introduction to Bigfoot Flow Cytometer – Instrumentation, Compensation & Panel Design
Lab 1B (Group B): Immune Cell Isolation Techniques – Magnetic Bead Separation & Density Gradient Protocols
|
| 03:30 PM – 03:45 PM |
Tea Break |
| 03:45 PM – 05:15 PM |
Hands-on Rotation (Switch A & B) |
| Day 1 – Hands-on Sessions |
|
Lab 1A: Introduction to Bigfoot Flow
Cytometer – Instrumentation,
Compensation & Panel Design |
Lab 1B: Immune Cell Isolation Techniques |
- Instrument orientation and software navigation
- Voltage optimization and compensation strategies
- Antibody panel design for multi-parametric analysis
- Quality control (QC) checks and standardization protocols
|
- Magnetic bead-based isolation (negative & positive selection)
- Density gradient centrifugation for peripheral blood mononuclear cell (PBMC) isolation
- Purity assessment and viability testing
- Protocol troubleshooting and optimization
|
Day 2: Translational Readiness, Immune Cell Sorting & Advanced Applications
| Time |
Programme |
| Session 3- Advanced Analysis & Clinical Translation |
|
| 09:30 AM – 10:00 AM |
Immune Profiling in Vaccine Research: Monitoring Immune Responses |
| 10:00 AM – 10:30 AM |
Quality Control, Standardization, and Regulatory Pathways in Flow Cytometry |
| 10:30 AM – 10:45 AM |
Panel Discussion |
| 10:45 AM – 11:00 AM |
Tea Break |
| 11:00 AM – 11:30 AM |
Investment Scenarios and Grant Opportunities for Translational Immunology |
| 11:30 AM – 12:00 PM |
Panel Discussion: Academia–Industry Collaborations for Immune Cell Technology Deployment |
| 12:00 PM – 01:00 PM |
Live Training on Flow Data Analysis using FlowJO Software |
| 01:00 PM – 02:00 PM |
Lunch |
| Lab Session 2 (Groups A & B Rotate) |
|
| 02:00 PM – 03:15 PM |
Lab 2A (Group A): Advanced Flow Cytometry – Multi-Parametric Immune Subset Profiling & Data Analysis
Lab 2B (Group B): Fluorescence-Activated Cell Sorting (FACS) – Isolation of Specific Immune Populations
|
| 03:15 PM – 03:30 PM |
Tea Break |
| 03:30 PM – 04:45 PM |
Hands-on Rotation (Switch A & B) |
| 04:45 PM – 05:30 PM |
Closing Ceremony – Feedback, Group Photo, Closing Remarks & Certificate Distribution |
| Day 2 – Hands-on Sessions |
|
| Participants will engage in structured, small-group practical sessions designed for experiential learning and competency building: |
|
| Lab 2A: Advanced Flow Cytometry – Multi-Parametric Immune Subset Profiling & Data Analysis |
Lab 2B: Fluorescence-Activated Cell Sorting (FACS) – Isolation of Specific Immune Populations |
- Gating strategies for immune cell identification (T cells, B cells, and NK cells)
- Fluorescent compensation and spillover correction.
- Bigfoot software tools for data visualization and statistical analysis Analysis of disease-specific immune phenotypes
|
- Cell sorting principles and instrument setup
- Purity and recovery optimization
- Downstream applications (RNA extraction, functional assays, culture)
- Safety protocols and ethical considerations
|
Participant Division:
Participants will be divided into two groups (A & B) and will rotate between lab stations on both days to ensure equal exposure and comprehensive learning
Target Participants:
- Early-career researchers, clinicians, and PhD scholars in biomedical sciences.
- Translational research teams from academia and industry.
Expected Outcomes:
- Basic Understanding of Immune Cell Profiling and Sorting
- Translational Research Competency in Immunology
- Hands-On Technical Proficiency and Analytical Skills
Support requirements:
- Logistical support for travel and accommodation of invited faculty
- Funding for workshop materials, antibodies, and reagents
- Technical support from ThermoFisher Scientific for instrument and software assistance
- Promotion through academic networks and institutional platforms
- Administrative support for participant registration and documentation
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