Dr. Krishna Das M. S. is an Assistant Professor at the Department of Pharmacology, Amrita School of Pharmacy, Kochi, India. Dr. Krishna Das MS obtained his M. Pharm. in Pharmacology from Mahatma Gandhi University, Kottayam, Kerala, India, in 2013 and Ph. D. in Pharmacology from Rajiv Gandhi University of Health Sciences, Bengaluru, India in 2019. His primary areas of focus are Autoimmunity, Neurodegeneration and Molecular Biology. He has 7 years of research and 2 years of teaching experience with 7 international publications.

Publications

Publication Type: Journal Article

Year of Publication Title

2018

Krishna Das MS, T, P., and S, M., “Role of Asiaticoside-A on axonal protection in experimental autoimmune encephalomyelitis.”, European Journal of Pharmaceutical and Medical Research. , vol. 5(11), 2018.[Abstract]


The aim of this study is to determine the action of Asiaticoside A against acute and chronic models of experimental autoimmune encephalomyelitis in mice. In this study, we investigated Asiaticoside A against acute and chronic models of EAE in mice. Asiaticoside A treated EAE animals shown reduced progression of the disease in both models. Gene expression studies revealed that the downregulation of IL-6, TNFα, IL-17a and CCL-5 in EAE mice were depending on the extend of treatment. The treatment with asiaticoside could not reverse the downregulation of NCAM1, FOXP3 and BDNF1 levels in EAE mice. Furthermore, histopathological studies revealed that the treatment with Asiaticoside A can able to control the demyelination, infiltrations and cellular changes in brain of EAE mice. Asiaticoside A delayed the EAE progression through the inhibition of inflammatory cytokines and chemokine and not by any other mechanisms of remyelination. The anti-inflammatory effect of this compound provides an alternative therapeutic management in multiple sclerosis. KEYWORDS: EAE, Asiaticoside, MS, Cytokines, Multiple Sclerosis

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2018

Krishna Das MS, T, P., and S, M., “Bacoside-A inhibits inflammatory cytokines and chemokine in experimental autoimmune encephalomyelitis. ”, Biomedicine & Pharmacotherapy , 2018.[Abstract]


Chronic inflammation of the myelin sheath is the crucial event behind the progression of multiple sclerosis (MS). Bacoside-A is one of the major constituents obtained from Bacopa monerii (L.) Wettst., and possess neuroprotective as well as anti-inflammatory actions. The current study explores the effect of Bacoside-A in acute and chronic models of Experimental Autoimmune Encephalomyelitis (EAE). The results indicate that the Bacoside-A treated mice produced a significant reduction in disease score compared to disease control in both models. The treatment with Bacoside-A downregulated the inflammatory cytokines (IL-6, IL-17a, and TNFα) and inflammatory chemokine CCL-5 in EAE mice. On the other hand, Bacoside-A treated mice showed a nonsignificant effect on promoting the expressions of NCAM, BDNF1, and FOXP3 in acute and chronic models of EAE. Histopathological analysis revealed that the Bacoside-A treated mice at a dose of 10 mg/kg exhibited a significant reduction in cellular infiltrations, cellular changes, and demyelination in cerebral tissues, but unable to protect at a higher dose in both models. In conclusion, Bacoside-A can able to inhibit the progression of EAE may be by the inhibition of inflammatory cytokines and chemokine evolved during active EAE.

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2018

Krishna Das MS and Tigari, P., “Bacoside-A Attenuated in Vitro Activation of Primary Astrocyte and Microglial Cultures”, European Journal of Pharmaceutical and Medical Research, vol. 5, pp. 337-341, 2018.[Abstract]


Activation of microglia and astrocytes is regarded as the initial phase in neuroinflammatory disorders like multiple sclerosis. The current study focus on the role of Bacoside-A on activated microglia and astrocyte. The action evaluated with LPS induced primary cultures of microglia and astrocyte. It reduced the production of NO and TNF- α in LPS stimulated microglia and astrocyte cultures. The present study emphasizes on the potential of Bacoside-A in neurodegenerative disorders like multiple sclerosis.

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2018

S. Maya, Prakash, T., and Krishna Das MS, “Assessment of neuroprotective effects of Gallic acid against glutamate-induced neurotoxicity in primary rat cortex neuronal culture”, Neurochemistry International, vol. 121, pp. 50-58, 2018.[Abstract]


Glutamate excitotoxicity plays a crucial role in the pathogenesis behind the development and progression of several neurodegenerative diseases. The study aimed to investigate the neuroprotective activity of Gallic acid (GA) against glutamate-induced neurotoxicity in primary rat cortex neurons (RCN). Treated the RCNs with GA 25 & 50 μg/ml for 2 h and later treated the cells with 100 μM glutamate (GLU) and incubated for 24 h at 37 °C. The results demonstrated that, the GA improved the antioxidant profile in the cortex neurons and inhibited the production of the proinflammatory cytokine. GA also maintained the Ca2+ homeostasis, IGF-1 expression, and protected the neurons from glutamate-induced neuronal toxicity. The neuroprotective activity of GA has further confirmed from the results of N-acetylaspartate and expression of microtubule-associated protein-2 expression. The reports suggest that, GA is significantly attenuated the glutamate-induced neurotoxicity and protected neurons from various chemical events that are involved in the pathogenesis of neurotoxicity.

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2018

Krishna Das MS and Tigari, P., “Asiaticoside counteracts the in vitro activation of microglia and astrocytes: Innuendo for multiple sclerosis”, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, vol. 107, pp. 303-305, 2018.[Abstract]


Background: During the development of Multiple Sclerosis (MS) there is a marked activation of microglia and astrocyte, leading to progressive inflammation and degeneration of myelin sheath which results in axonal loss and neuron damage.

Purpose: In this study, we have explored the action of Asiaticoside A against the activated microglia and astrocytes.

Methods: Primary microglia and astrocyte cultures were used for the study and the activity were evaluated using cell proliferation assay, nitrate assay and TNFα estimation using ELISA.

Results: Asiaticoside A inhibited the production of nitric oxide and TNFα in LPS activated primary microglia and astrocyte cultures.

Conclusion: This study suggests that Asiaticoside A may be effective against the progression of MS.

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2016

S. Maya, Tigari, P., Krishna Das MS, and Goli, D., “Multifaceted effects of aluminium in neurodegenerative diseases: A review”, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, vol. 83, pp. 746-754, 2016.[Abstract]


Aluminium (Al) is the most common metal and widely distributed in our environment. Al was first isolated as an element in 1827, and its use began only after 1886. Al is widely used for industrial applications and consumer products. Apart from these it is also used in cooking utensils and in pharmacological agents, including antacids and antiperspirants from which the element usually enters into the human body. Evidence for the neurotoxicity of Al is described in various studies, but still the exact mechanism of Al toxicity is not known. However, the evidence suggests that the Al can potentiate oxidative stress and inflammatory events and finally leads to cell death. Al is considered as a well-established neurotoxin and have a link between the exposure and development of neurodegenerative diseases, including Amyotrophic Lateral Sclerosis (ALS), Alzheimer's disease (AD), dementia, Gulf war syndrome and Parkinsonism. Here, we review the detailed possible pathogenesis of Al neurotoxicity. This review summarizes Al induced events likewise oxidative stress, cell mediated toxicity, apoptosis, inflammatory events in the brain, glutamate toxicity, effects on calcium homeostasis, gene expression and Al induced Neurofibrillary tangle (NFT) formation. Apart from these we also discussed animal models that are commonly used for Al induced neurotoxicity and neurodegeneration studies. These models help to find out a better way to treat and prevent the progression in Al induced neurodegenerative diseases.

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2014

Krishna Das MS and Harindran, J., “Antiarthritic potential of Ocimum gratissimum L. in collagen induced arthritic Sprague-Dawley rats”, Biomedicine & Aging Pathology, vol. 4, pp. 191-196, 2014.[Abstract]


Aim Rheumatoid arthritis is a systemic inflammatory disease, which mainly affects joints but can also affect other organs. Ocimum gratissimum L. (Lamiaceae) is a herb, which is reported to have anti-inflammatory activity. So the present investigation was designed to evaluate antiarthritic potential of O. gratissimum in Collagen induced arthritis (CIA) in laboratory animals. Materials and methods Fresh leaves were extracted using 95% ethanol by maceration process. The extracts were tested against collagen induced female Sprague-Dawley rats. Arthritis assessment was carried out on the basis of parameters including arthritic score, body weight and paw volume. On day 41, animals were evaluated for biochemical parameter, radiological parameter and histological parameters. Results Treatment with 500mg/kg of EEOG significantly reduced arthritic score, paw volume and almost restored body weight of arthritic animals compared to control animals. Meanwhile, the treatment significantly attenuated biochemical, radiological and histopathological alteration induced by CIA. Conclusion EEOG can able to nullify most of the ill effects produced by CIA in rats. However, further studies are needed to carry out identification, isolation of active fractions of EEOG to unravel the mechanism of drug.

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Publication Type: Presentation

Year of Publication Title

2017

Krishna Das MS, “Role of triterpenoid glycoside on axonal protection in multiple sclerosis and experimental autoimmune encephalomyelitis, at Fifth International Conference on Drug Discovery India 2017 by SELECTBIO, Bengaluru, India, September 14-15”. 2017.

Faculty Research Interest: