Course

1. Isolation of Plasmid DNA by Alkaline lysis method.
2. Quantification of DNA.
3. Detection of Plasmid DNA by Agarose gel electrophoresis.
4. Restriction Digestion Analysis.
5. Competent cell preparation.
6. Transformation and Efficiency of competent cells.
7. SDS PAGE
8. Polymerase Chain Reaction.
9. Isolation of Genomic DNA.
10. Calibration of pipettes.

The students will learn the theoretical and practical aspects of key molecular biology experiments like Plasmid DNA isolation, Restriction digestion, PCR, Competent cell preparation, Transformation, SDS-PAGE etc. Hands on experience will be given to all the students.?

COURSE OUTCOMES:

After completing the course, students shall be able to

CO1: Students will discover how to isolate plasmid DNA

CO2: Students will identify a plasmid experimentally.

CO3: Students will illustrate restriction digestion followed by its mapping

CO4: Students will design PCR conditions and perform PCR.

CO5: Students will show competent cell preparation and transformation.

CO6: Students will assemble and perform SDS PAGE.

1. Joseph Sambrook, David William Russell “Molecular cloning”. 3rd Edition, CSHL Press, 2001.