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Course Detail

Course Name Biochemistry Lab
Course Code BIO 482
Program M. Sc. in Biotechnology, M. Sc. in Microbiology
Semester One
Credits two
Year Taught 2019


Preparation of Laboratory Solutions and Buffers [problems and Calculations],  Separation of plant pigments by column chromatography ,Thin layer chromatography , Preparation of standard curve  for the Enzyme Assay, Effect of substrate concentration on Enzyme kinetics and determination of Km and Vmax,  Estimation of protein and specific activity of the enzyme , Structural studies of phycobiliproteins from spirulina using  spectroscopic techniques , Protein purification [Ammonium sulphate precipitation, Affinity Chromatography and SDS PAGE],  Lab activity

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