Course

Unit I

Introduction to Biosensor
Classification based on the signal transduction and biorecognition element. Enzymatic and non-enzymatic sensors, DNA and protein-based sensors-immunosensors.

Unit II

Biosensing using nanomaterials
Concepts of surface to volume ratio, quantum confinement, surface plasmon resonance, fluorescence, chemiluminescence and electroluminescence and FRET in biosensing. Application of metal, semiconducting quantum dots, carbon nanotubes, graphene and carbon dots in biosensing.

Unit III

Electrochemical principle in biosensing
Principles of potentiometry, voltammetry, amperometry and impedimentary in biosensing. Principle, fabrication and working of optical, electrochemical biosensors. Construction and working of potentiometric, amperometric and impedimetric sensors. Development and applications of piezoelectric sensors.

Unit IV

Optical and electrochemical sensors for glucose, vitamins, cholesterol, dopamine, nitric oxide, nitrates, and pesticides. Biocompatibility of sensors.

Unit V

Biochips and Wearable Devices
lab-on-a-chip – fabrication of microfluidics- lithography, wearable sensors, epidermal electronic system, lab- on-skin-devices.

• CO1: Explain the classification of biosensors based on signal transduction and biorecognition element.
• CO2: Explain the significance of physicochemical properties of nanomaterials to their application as sensors.
• CO3: Describe the operational principle and development of potentiometric, amperometric, impedimetric and piezoelectric sensors.
• CO4: Describe the different optical optical and electrochemical sensors for detection of important chemical species.
• CO5: Describe the fabrication of sensors in the form of biochips and wearable devices.

References

1. Xueji Zhang, Huangxian Ju, Joseph Wang, “Electrochemical Sensors, Biosensors and Their Biomedical Applications”, Elsevier, 2008.
2. Joseph Wang, “Analytical Electrochemistry”, Wiley, 2006
3. Huangxian Ju, Xueji Zhang, Joseph Wang, “Nano Biosensing: Principles, Development and Application”, Springer, 2011.
4. Peter Grundler, “Chemical Sensors – An Introduction for Scientists and Engineers”, Springer – Verlag, Berlin Heidelberg, 2007.
5. Arben Merkoci,“Biosensing using nanomaterials” Wiley, 2009