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Course Detail

Course Name Clinical Biochemistry and Proteomics
Course Code 25MMD512
Program M. Sc. Molecular Medicine(MLM)
Semester 2
Credits 3
Campus Kochi

Syllabus

Unit 1

Biochemical mechanisms leading to generation of essential biological molecules for cellular architecture generation and preservation for maintaining cellular physiology. Significance of carbohydrate and fatty acid metabolism in this process. Glycolysis; TCA cycle, Oxidative Phosphorylation – Energetics and its regulation; Pentose phosphate Pathway; Glycogen Metabolism; Gluconeogenesis pathway and significance; Biosynthesis of fatty acids; Oxidation of fatty acids – Beta oxidation, alpha oxidation.

Unit 2

Structures & function of enzymes, mechanism of action of enzymes and its regulation; Kinetics of enzyme catalyzed reactions, Michaelis-Menten equation, Importance of Vmax, Km; Enzyme inhibition and activation.

Unit 3

Concept and scope of clinical biochemistry in the context of cancer. Control of the blood glucose and associated clinical diseases in the context of diabetes.

Unit 4

Proteome and proteomics research, how it is different from genomics; different types of proteomics, significance of sample preparation in proteomics, significance of choosing different methods for proteome analysis, gel-free and gel-based proteome analysis, labelled and label-free quantitative proteomics.

Unit 5

Mass spectrometry and its significance in modern science and medicine. Principles of mass spectrometry; protein identification using mass spectrometry, protein fragmentation; peptide enrichment and separation; ionization and its importance; Time of Flight, MS/MS analysis, types of mass analyzers, peptide fragmentation and peptide sequencing; identification of proteins using search engines/programs; accuracy of identified proteins with respect to protein identity, significance of mass spectrometry in clinics, clinical proteomics and examples of clinical proteomics

Objectives and Outcomes

Course Outcome

CO1: Understand overall concept of cellular metabolism, energy storage and release, enzymes and their regulation

CO2: Understand glucose homeostasis (pathways and hormonal regulation); glycogen metabolism, gluconeogenic pathway, fat metabolism.

CO3: Understand the basic concepts and principles of clinical biochemistry and molecular mechanism of some common biochemical disorders

CO4: Understand the basic concepts of proteome, mass spectrometry and protein identification using database search engines.

CO5: Discuss how proteomics can contribute to a clinical setting.

Programme Outcomes (PO) (As given by NBA and ABET)

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

 

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

CO1

3

1

1

1

1

CO2

3

1

1

1

CO3

3

2

1

2

2

1

CO4

3

1

2

2

3

1

CO5

3

1

2

3

3

2

1

Program Specific Outcomes. (PSO)

PSO 1 – Biochemical organization and cellular complexity in function

PSO 2 – Biomolecules in Medicine

PSO 3 – Molecular dysregulation in diseases

PSO 4 – Molecular technology in diagnosis and therapy

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in Medicine

PSO 7 – Nanoscale entities and its significance in Medicine

PSO 8 – Tissue architecture engineering in Medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and biological data use

 

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

CO1

3

2

1

CO2

3

2

1

CO3

1

3

3

2

1

CO4

1

1

3

3

CO5

2

1

3

3

Evaluation Pattern

Evaluation Pattern: 50+50 = 100

Internal Assessment – 50% 

Periodical 1 

Exam 

20% 

Periodical 2 

Exam 

20% 

Continuous Assessment 

Assignment/Test/Quiz

10% 

 

50%

End Semester Examination- 50% 

Theory Exam 

50% 

 
 

50%

Total

100%

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