Back close

Purification of Fibronectin Using Gelatin-Polymer Conjugates

Start Date: Saturday, Jan 01,2011

End Date: Monday, May 30,2011

School: School of Biotechnology

Thematic Area: Biotech

Project Incharge:Dr. Sanjay Pal
Co-Project Incharge:Veena, Uma Maheswari, Suvitha, Archana Kini, Swathy Marrar, Arjun KV, Uma VK, Jyostna T, Lekshmi Sankar, Vignesh Narayan H
Purification of Fibronectin Using Gelatin-Polymer Conjugates

Fibronectin is one of the extraceullar proteins in vertebrates generated by alternative splicing, with twenty more possible isoforms. This project involves a survey of current fibronectin literature, followed by an account of our experiments to purify fibronectin from plasma using gelatin affinity chromatography. Gelatin was conjugated/copolymerized with agarose or polyacrcylamide and their efficacies of the purification were compared by SDS-PAGE followed by densitometric scanning and analysis by ImageJ software.

Related Projects

Expression, Purification And Refolding Of Recombinant Amidase In Escherichia Coli
Expression, Purification And Refolding Of Recombinant Amidase In Escherichia Coli
Schiff’s Base Derivative Of Chitosan: A Potential Matrix For Metalloprotein Enrichment
Schiff’s Base Derivative Of Chitosan: A Potential Matrix For Metalloprotein Enrichment
Fibronectin Purification and Fragmentation
Fibronectin Purification and Fragmentation
Development of peptide inhibitors against functional components of snake venom; a new approach
Development of peptide inhibitors against functional components of snake venom; a new approach
Folding of the Vector (pCDH-CMV-MCS-EF1-puro) into Predefined Shape Using 20mer Staples
Folding of the Vector (pCDH-CMV-MCS-EF1-puro) into Predefined Shape Using 20mer Staples
Admissions Apply Now