Project

Purification of Fibronectin Using Gelatin-Polymer Conjugates

Fibronectin is one of the extraceullar proteins in vertebrates generated by alternative splicing, with twenty more possible isoforms. This project involves a survey of current fibronectin literature, followed by an account of our experiments to purify fibronectin from plasma using gelatin affinity chromatography. Gelatin was conjugated/copolymerized with agarose or polyacrcylamide and their efficacies of the purification were compared by SDS-PAGE followed by densitometric scanning and analysis by ImageJ software.