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Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : International Journal of Biological Macromolecules
Source : International Journal of Biological Macromolecules, Elsevier B.V., Volume 110, p.558-566 (2018)
Campus : Kochi
School : Center for Nanosciences
Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences
Department : Nanosciences and Molecular Medicine, Nanosciences
Year : 2018
Abstract : Qualitative 2D gel-electrophoresis (2DE) protein profiling for osteoarthritis (OA) and rheumatoid arthritis (RA) is challenging because of selective protein loss due to discrepancies in protein precipitation methodologies. Thus, we aimed at developing qualitative proteinrepresentation from OA/RA articular cartilage without protein precipitation towards identification of clinically relevant proteins. Chondroitinase digested human articular cartilages from RA patients were subjected to protein extraction using guanidinium hydrochloride (GuHCl) or 8 M urea with 10 or 2% ASB-14-4 or 0.45 M urea with 2% ASB-14-4 with cetylpyridinium chloride (CPC). The GuHCl extract is further protein precipitated with acetone or ammonium acetate-methanol or centricon-fractionated using 100 kDa cut filters and protein precipitated using ethanol. Processed extracts were subjected to 2DE to identify protein profiles. Poor proteins representations were observed in 2D gels with protein precipitated samples compared to qualitative protein representations seen in 2D gels of 0.45 M urea and 2%ASB-14-4 extraction procedure reproducibly. The strategy circumventing protein precipitation generated qualitative 2D gels. RA vs OA gel comparison showed elevated prolargin levels in RA with biglycan levels remaining unaltered. Up regulation of prolargin in RA suggests the likelihood of an adaptive mechanism to control the increased osteoclastogenesis in RA and may have therapeutic value in controlling the disease.
Cite this Research Publication : Vinod Soman Pillai, Rameshwari R. Kundargi, Fabia Edathadathil, Shantikumar V Nair, Thilak, J., Roshini Anney Mathew, Xavier, T., Padmanabha Shenoy, and Krishnakumar N. Menon, “Identification of Prolargin Expression in Articular Cartilage and its Significance in Rheumatoid Arthritis Pathology”, International Journal of Biological Macromolecules, vol. 110, pp. 558-566, 2018.