Course

Unit I

Enzymes
Enzyme nomenclature, Enzyme commission numbers, Enzymes in bioanalytical chemistry, Enzyme kinetics – Enzyme activators, Enzyme inhibitors.

Unit II

Quantification of Enzymes and their Substrates
Instrumental methods, optical detection – absorbance, Fluorescence, Luminescence, Nephlometry Electrochemical detection – amperometry, potentiometry, conductometry, other detection methods – radiochemical, manometry, calorimetry.

Unit III

Immobilized Enzymes
Immobilization methods – nanopolymerizing covalent immobilization, Crosslinking with bifunctional reagents. Properties of immobilized enzymes, immobilized enzyme reactions, theoretical treatment of packed bed enzyme reactors.

Unit IV

Antibodies
Structural and functional properties of antibodies, polyclonal and monoclonal antibodies. Antibody-antigen interactions, analytical application of secondary antibody-antigen.

Unit V

Biosensors
Response of enzyme-based biosensors, examples of Biosensor Configuration, Ferrocene-mediated amperometric glucose sensor, potentiometric biosensor for phenyl acetate, potentiometric immunosensor for digoxin, optical biosensor for glucose-based on fluorescence energy transfer, piezoelectric sensor for nucleic acid detection, enzyme thermistors.

• CO1: Explain enzyme classification, nomenclature and effect of activators and inhibitors on enzyme action.
• CO2: Describe and compare instrumental techniques for quantification of enzymes and their substrates.
• CO3: Explain the strategies for enzyme immobilization and the properties of immobilized enzymes.
• CO4: Describe the structure and function of antibodies and interpret antibody-antigen interactions for analytical and diagnostic applications.
• CO5: Describe working principles and configurations of enzyme based biosensors for chemical and biological applications.

Textbooks

1. Susan R. Mikkelsen and Eduardo Corto´n ‘Bioanalytical Chemistry’, 1^stedition, Wiley Interscience, 2003.
2. Andres Manz, Nicole Pamme and Dimitri Lossifidis, ‘Bioanalytical Chemistry’, World Scientific Publishing Company,  2004.

Reference

1. Robert W. Cattrall, ‘Chemical Sensors’, Oxford University Press, 1997