Course

Unit 1

Introduction to Genetics: Genes, chromosomes & heredity, DNA as genetic material. Mendelian principles, extension and variation of Mendelism, problem solving for both mendelian and non mendelian crosses.

Unit 2

Chromosomal basis of Heredity: Variation in chromosome number & structure, genetic basis of sex determination in selected organisms. Population and Evolutionary Genetics. Extrachromosomal inheritance

Unit 3

Linkage and Crossing-over: Mechanism of chromosomal linkage and crossing-over. Molecular mechanisms of recombination. Genetic and cytological mapping. Genetic markers and distances. Pedigree analysis.

Unit 4

Mutations and DNA repair: Classification and source of mutations. Polymorphisms. Types of DNA repair mechanisms. Transposable elements and their classification.

Unit 5

Genome editing: homologous recombination, zinc-finger nucleases, TALENS, CRISPR-Cas9, site directed mutagenesis.

LEARNING OBJECTIVES:

Genetics is the study of heredity and genes. The aim of this course is to strengthen the Mendelian principles along with other molecular genetics topics like recombination, pedigree analysis, transposons. This course will help students to venture into the different areas of biomedical sciences.

COURSE OUTCOMES:

After completing the course, students shall be able to

CO1. Students will explain the basic concept of Mendelian principles and apply it in different genetic experiments. This would help the students to solve the majority of genetic problems.

CO2. Students will analyze the deviations from the standard mendelian laws in a few cases and learn the mechanisms.

CO3. Students will identify the underlying genetic mechanisms that regulate sex determination and clinical cases leading into chromosome abnormalities.

CO4. Students will describe the principles of linkage, recombination and chromosome mapping to establish the physical and genetic connection between two neighboring genes.

CO5. Students will explain different types of mutations and how DNA repair mechanisms restore the integrity following the DNA damage. 

CO6. Describe the process of gene editing using different methods like homologous recombination, zinc finger nucleases, TALENS, CRISPR-Cas9, site directed mutagenesis, identify the technical and ethical barriers of gene editing.

REFERENCES:1. Genetics ? 6th Edition ? Snustad& Simmons ? Wiley, 2011.2. Concepts of Genetics ? 10th Edition ? Klug et al ? Pearson, 2011.3. E.J.Gardner, M.J. Simmons and D.P. Snustad.1991″Principles of Genetics”. Eighth edition John Wiley.4. D.L. Hartl and E.W. Jones. ?Genetics, Analysis of Genes and Genomes?. Sixth edition. Jones and Bartlett Publishers. 2004