| Course Name | Clinical Biochemistry and Proteomics |
| Course Code | 25MMD512 |
| Program | M. Sc. Molecular Medicine(MLM) |
| Semester | 2 |
| Credits | 3 |
| Campus | Kochi |
The course intends to provide a basic understanding of the biochemical reactions; role of macromolecules and enzymes that govern the biochemical transformations and biochemical mechanisms responsible for common biochemical disorders; mass-spectrometry based proteomics for protein identification and quantitation, application of proteomics in the clinics for aiding in diagnosis, prognosis and treatment of diseases.
Biochemical mechanisms leading to generation of essential biological molecules for cellular architecture generation and preservation for maintaining cellular physiology. Significance of carbohydrate and fatty acid metabolism in this process. Glycolysis; TCA cycle, Oxidative Phosphorylation – Energetics and its regulation; Pentose phosphate Pathway; Glycogen Metabolism; Gluconeogenesis pathway and significance; Biosynthesis of fatty acids; Oxidation of fatty acids – Beta oxidation, alpha oxidation.
Structures & function of enzymes, mechanism of action of enzymes and its regulation; Kinetics of enzyme catalyzed reactions, Michaelis-Menten equation, Importance of Vmax, Km; Enzyme inhibition and activation.
Concept and scope of clinical biochemistry in the context of cancer. Control of the blood glucose and associated clinical diseases in the context of diabetes.
Proteome and proteomics research, how it is different from genomics; different types of proteomics, significance of sample preparation in proteomics, significance of choosing different methods for proteome analysis, gel-free and gel-based proteome analysis, labelled and label-free quantitative proteomics.
Mass spectrometry and its significance in modern science and medicine. Principles of mass spectrometry; protein identification using mass spectrometry, protein fragmentation; peptide enrichment and separation; ionization and its importance; Time of Flight, MS/MS analysis, types of mass analyzers, peptide fragmentation and peptide sequencing; identification of proteins using search engines/programs; accuracy of identified proteins with respect to protein identity, significance of mass spectrometry in clinics, clinical proteomics and examples of clinical proteomics
Course Outcome
CO1 To understand patterns of inheritance and laws of heredity at molecular levels
CO2 To understand about chromosomes and their transmission during cell divisions, genetic linkage and mapping in Eukaryotes, variations in chromosome structure and number, and chromosome organization and molecular structure
CO3 To comprehend various modes of epigenetic regulation on gene expression in
Eukaryotes and roles of noncoding RNA in gene regulation
CO4 To learn about genetic principles underlying medical, immune and developmental aspects
CO5 To gather knowledge of complex and quantitative traits, polygenic
Inheritance, population genetics, phylogenic trees and molecular evolution
Program Outcomes (PO) (As given by NBA and ABET)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
| c | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| C O | ||||||||||||
| CO 1 | 3 | 3 | 3 | 3 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 3 |
| CO 2 | 3 | 2 | 2 | 2 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 3 |
| CO 3 | 3 | 2 | 2 | 2 | 3 | 3 | 3 | 0 | 0 | 0 | 0 | 3 |
| CO 4 | 3 | 1 | 1 | 2 | 3 | 3 | 3 | 0 | 0 | 0 | 0 | 3 |
| CO 5 | 3 | 3 | 3 | 3 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 3 |
0 – No affinity; 1 – low affinity; 2 – Medium affinity; 3 – High affinity
Program Specific Outcomes. (PSO)
PSO 1 – Biochemical organization and cellular complexity in function
PSO 2 – Biomolecules in Medicine
PSO 3 – Molecular dysregulation in diseases
PSO 4 – Molecular technology in diagnosis and therapy
PSO 5 – Cell based approaches in diagnosis and therapy
PSO 6 – Microorganisms in Medicine
PSO 7 – Nanoscale entities and its significance in Medicine
PSO 8 – Tissue architecture engineering in Medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and biological data use
| C | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 |
| C O | ||||||||||
| CO 1 | 3 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 1 |
| CO 2 | 3 | 1 | 3 | 3 | 3 | 0 | 0 | 3 | 0 | 3 |
| CO 3 | 3 | 3 | 3 | 3 | 2 | 0 | 0 | 2 | 0 | 3 |
| CO 4 | 3 | 2 | 3 | 3 | 1 | 0 | 0 | 2 | 3 | 0 |
| CO 5 | 3 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 3 |
TEXT BOOKS:
REFERENCE:
Lehniger Principles of biochemistry 8th Edition (2021), Publisher: WH Freeman, Authors Michael Cox, David L. Nelson.
Evaluation Pattern: 50+50 = 100
| Internal Assessment – 50% | ||
| Periodical 1 | Exam | 20% |
| Periodical 2 | Exam | 20% |
| Continuous Assessment | Assignment/Test/Quiz | 10% |
| 50% | ||
| End Semester Examination- 50% | ||
| Theory Exam | 50% | |
| 50% | ||
| Total | 100% | |
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