Pre-requisites: Undergraduate level basic biology, biotechnology
Total number of classes: 30
| Course Name | Recombinant DNA Technology |
| Course Code | 25MMD543 |
| Program | M. Sc. Molecular Medicine(MLM) |
| Credits | 3 |
| Campus | Kochi |
Pre-requisites: Undergraduate level basic biology, biotechnology
Total number of classes: 30
Restriction enzymes and cloning vectors: Host controlled restriction modification,
Restriction endonucleases – types and classification, modifying enzymes used in molecular cloning – methylases, polymerases, ligases, kinases, phosphatases and nucleases
Vectors and Hosts: Vectors for cloning, expression vectors (plasmids, lambda phage vectors, cosmids, BAC & YAC); Host organisms used in r-DNA technology: E. coli, Yeast, Insect cells as model organisms.
Cloning, Construction of Genomic and cDNA libraries: Introduction to cloning
(conventional & recombination based: traditional cloning, TA cloning, Gateway cloning etc), vGene transfer techniques: Transformation, transfection and transduction, Strategies for construction of genomic libraries, Strategies for construction of cDNA libraries.
Selection and characterization of recombinant clones: Genetic Selection- insertional
inactivation and alpha complementation, Labeling of nucleic acids, Immunological probes, Selection of recombinant clones-hybridization techniques, colony hybridization & library screening, hybrid arrest & hybrid release translation, DNA sequencing methods, DNA arrays.
Advanced techniques and applications: Genome editing- CRISPR-Cas system, TALENs & ZFNs, Site directed mutagenesis and RNA interference, Recombinant vaccines, Recombinant antibodies (Fab, scFv, sdAb), Disease diagnosis, Gene therapy- technologies, applications and regulations Next generation sequencing- principle, types and applications.
Course Outcome
CO1 Understand the fundamentals of molecular cloning
CO2 Understand the technical know-how on versatile techniques in recombinant DNA technology.
CO3 Gain knowledge of tools and strategies used in molecular cloning
CO4 Understand how to select and characterize recombinant clones
CO5 Understand basic and advanced molecular cloning concepts, applications and techniques
Programme Outcomes (PO) (As given by NBA and ABET)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
| c | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| C O | ||||||||||||
| CO 1 | 3 | – | 2 | – | 2 | – | – | 2 | – | – | – | 2 |
| CO 2 | 3 | 1 | 2 | 2 | 3 | – | – | – | – | – | – | 1 |
| CO 3 | 3 | 2 | 1 | 1 | 3 | 2 | – | – | – | – | – | 1 |
| CO 4 | 3 | 2 | 3 | 3 | – | 1 | – | – | – | – | – | 1 |
| CO 5 | 3 | – | – | 2 | 3 | 1 | – | – | – | – | – | 1 |
Program Specific Outcomes. (PSO)
PSO 1 – Biochemical organization and cellular complexity in function
PSO 2 – Biomolecules in Medicine
PSO 3 – Molecular basis of disease
PSO 4 – Molecular technology in diagnosis and therapy
PSO 5 – Cellular based approaches in diagnosis and therapy
PSO 6 – Microorganisms in Medicine
PSO 7 – Nanoscale entities and its significance in Medicine
PSO 8 – Tissue architecture engineering in Medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and biological data use
| – | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 |
| C O | ||||||||||
| CO 1 | – | – | – | 3 | 1 | 2 | – | – | – | 1 |
| CO 2 | – | – | – | 3 | – | _ | – | – | – | 1 |
| CO 3 | – | – | – | 3 | 2 | 1 | – | – | – | 1 |
| CO 4 | – | – | – | 3 | – | – | – | – | – | – |
| CO 5 | – | – | – | 3 | – | – | – | – | – | – |
Evaluation Pattern: 50+50 = 100
| Internal Assessment – 50% | ||
| Periodical 1 | Exam | 20% |
| Periodical 2 | Exam | 20% |
| Continuous Assessment | Assignment/Test/Quiz | 10% |
| 50% | ||
| End Semester Examination- 50% | ||
| Theory Exam | 50% | |
| 50% | ||
| Total | 100% | |
TEXT BOOKS:
REFERENCE
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