Course

‘Pharmaceutical Analysis – Practical’ is a course offered in the third year of Pharm. D. program at School of Pharmacy, Health Sciences campus, Amrita Vishwa Vidyapeetham.

Course Duration: 3 Hrs./Week

List of Experiments:

Title

1. Separation and identification of Amino Acids by Paper Chromatography.
2. Separation and identification of Sulpha drugs by TLC technique.
3. Effect of pH and solvent on the UV spectrum of given compound.
4. Comparison of the UV spectrum of a compound with that of its derivatives.
5. Determination of dissociation constant of indicators using UV-Visible spectroscopy.
6. Conductometric titration of mixture of acids with a strong base.
7. Potentiometric titration of a acid with a strong base.
8. Estimation of drugs by Fluorimetric technique.
9. Study of quenching effect in fluorimetry.
10. Colourimetric estimation of Supha drugs using BMR reagent.
11. Simultaneous estimation of two drugs present in given formulation.
12. Assay of Salicylic Acid by colourimetry.
13. Determination of Chlorides and Sulphates in Calcium gluconate by Nepheloturbidimetric Method.
14. Determination of Na/K by Flame Photometry.
15. Determination of pKa using pH meter.
16. Determination of specific rotation.
17. Comparison of the IR spectrum of a compound with that of its derivatives.
18. Demonstration of HPLC.
19. Demonstration of HPTLC.
20. Demonstration of GC-MS.
21. Demonstration of DSC.
22. Interpretation of NMR spectra of any one compound.

Reference Books:

1. Text Book of Pharm. Analysis by Higuchi. T and Hasen. E. B., New York Inter Science Publishers.
2. Quantitative Pharma. Analysis by Jenkins, The Blakiston division, New York.
3. Quantitative Drug Analysis, by Garrot. D, Chapman & Hall Ltd., London.
4. Undergraduate Instrumental Analysis by James. E., CBS Publishers.
5. Instrumental Analysis by Willard and Merritt, EWP, East West Press Ltd., Delhi/Madras.
6. Pharm Analysis by Skoog and West, Sounders Manipal College Publishing.
7. Text Book of Chemical Analysis, by A.I.Vogel, ELBS with Macmillan press, Hampshire.
8. Textbook of Pharm. Analysis by K.A.Connors, John Wiley & Sons, New York, Brisbane, Singapore.
9. Textbook of Pharm. Analysis (Practical) by Beckett & Stenlake, CBS Publishers, Delhi.
10. Textbook of Drug Analysis by P.D. Sethi., CBS Publishers, Delhi.
11. Spectroscopy by Silverstein, John & Wiley & Sons. Inc., Canada & Singapore.
12. How to practise GMP-A Plan for total quality control by P.P. Sharma, Vandana Publications, Agra.
13. The Science & Practice of Pharmacy by Remington Vol-I & II, Mack Publishing Co. Pennsylvania.
14. TLC by Stahl, Spring Verlay..
15. Text Book of Pharm. Chemistry by Chatten, CBS Publications.
16. Spectroscopy by William Kemp, ELBS with Macmillan Press, Hampshire
17. I.P.-1996, The Controller of Publications, New Delhi.
18. BPC- Dept. of Health, U.K. for HMSO.
19. USP – Mack Publishing Co., Easton, PA.
20. The Extra Pharmacopoeia – The Pharm. Press, London.