The silver nanoparticles (AgNPs) synthesized using leaf extracts as reducing and stabilizing agent are already reported and evaluated for antibacterial activity against drug resistant bacteria isolates. The effect of extract concentration, synthesis time, pH and temperature on the reaction rate and the shape of the Ag nanoparticles are investigated. The nature of AgNPs synthesized was characterized by UV–vis spectroscopy, Dynamic light scattering method and Transmission electron microscopy (TEM). The particle size was estimated around 100 nm. The antibacterial potential of synthesized AgNPs was compared with that of aqueous leaf extracts by well diffusion method. The AgNPs at 0.1-30 μg/ml concentration significantly inhibited bacterial growth against multi drug resistant Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli). The synthesized Ag NPs has been developed by using the fresh leaf extract of Bauhinia Acuminata, Cynodon dactylon, Gracinia cambogia and it showed minimum inhibition activity at 0.5 μl.
