| Course Name | Molecular Biology Lab |
| Course Code | 25CLG581 |
| Program | M. Sc. Clinical Genomics |
| Semester | 1 |
| Credits | 2 |
| Campus | Kochi |
Isolation of chromosomal DNA from Escherichia coli; Isolation of chromosomal DNA from human blood; Isolation of plasmid DNA from Escherichia coli; Nucleic acid quantification; Polymerase chain reaction (PCR); Agarose gel electrophoresis; Restriction digestion, Restriction fragment length polymorphism (RFLP), RNA isolation from human blood; cDNA synthesis, Reverse Transcriptase PCR, Real time PCR; DNA sequencing.
Pre-requisites: Basic understanding of DNA chemistry and DNA biology
Total number of classes: 30
Course Outcome
CO1 Explain the principles of the DNA & RNA isolation methods, PCR, agarose gel electrophoresis, sequencing methods.
CO2 Can isolate DNA, RNA, plasmids.
CO3 Can perform PCR, cDNA synthesis, RT-PCR, Real-time PCR, and sequencing.
CO4 Can follow general safety routines for laboratory work in molecular biology.
CO5 Can plan experimental work based on a protocol and critically evaluate and discuss experimental results.
*Program Outcomes (PO)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
CO–PO Mapping Table:
| C O | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| CO 1 | 3 | 2 | 2 | 1 | 3 | 1 | – | 1 | 3 | – | – | 1 |
| CO 2 | 3 | 2 | 3 | 3 | 3 | 1 | – | 1 | 3 | – | – | 1 |
| CO 3 | 3 | 2 | 3 | 3 | 3 | 1 | – | 1 | 3 | – | – | 1 |
| CO 4 | 2 | 3 | 3 | 2 | 2 | 1 | – | – | – | – | – | 1 |
| CO 5 | 3 | 3 | 1 | 2 | 3 | 2 | – | – | – | – | – | 1 |
Program Specific Outcomes (PSO):
PSO1. Apply fundamental molecular biology principles to interpret clinical genomic data.
PSO2. Use molecular techniques (e.g., PCR, RT-PCR, sequencing) to detect genetic mutations and biomarkers.
PSO3. Analyze genotype-phenotype correlations in inherited and acquired disorders.
PSO4. Identify pathogenic variants from NGS data and interpret their clinical relevance.
PSO5. Correlate molecular pathways with disease mechanisms and therapeutic targets.
PSO6. Develop and validate diagnostic assays based on molecular biology principles.
PSO7. Utilize molecular biology to support pharmacogenomic profiling and therapy optimization.
PSO8. Integrate multi-omic data (genomic, transcriptomic, epigenomic) for personalized health solutions.
PSO9. Apply molecular knowledge to cancer genomics, infectious diseases, and rare genetic disorders.
PSO10. Translate molecular discoveries into clinical interventions through evidence-based practice.
CO-PSO Mapping Table:
| COs | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 |
| CO1 | 3 | 3 | 2 | 2 | 2 | 3 | 2 | 2 | 1 | 2 |
| CO2 | 2 | 3 | 1 | – | – | 3 | – | – | 1 | 1 |
| CO3 | 3 | 3 | 2 | 3 | 2 | 3 | 2 | 2 | 2 | 3 |
| CO4 | 1 | 2 | – | – | – | 2 | – | – | 1 | 1 |
| CO5 | 3 | 3 | 2 | 3 | 3 | 3 | 2 | 2 | 2 | 3 |
Evaluation Pattern: 30+70 = 100
| Internal Assessment – 30% | ||
| Records | Evaluation | 30% |
| 30% | ||
| End Semester Examination – 50% | ||
| Practical | Exam | 60% |
| Assessment | Viva | 10% |
| 70% | ||
Textbooks
References
Gene Therapy Technologies, Applications and Regulations: From Laboratory to Clini. Anthony Meager 1999 John Wiley & Sons, Ltd.
DISCLAIMER: The appearance of external links on this web site does not constitute endorsement by the School of Biotechnology/Amrita Vishwa Vidyapeetham or the information, products or services contained therein. For other than authorized activities, the Amrita Vishwa Vidyapeetham does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this web site.
