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Course Detail

Course Name Flow cytometry and applications
Course Code 25NBT533
Program M. Sc. Nanobiotechnology (NBT)
Credits 3
Campus Kochi

Syllabus

Unit 1

Introduction to Flow Cytometry, Overview of flow cytometry and its historical development, Basic components of a flow cytometer: fluidics, optics, and electronics, Principles of fluorescence and fluorochrome selection.

Unit 2

Instrumentation and Operation, Detailed study of flow cytometer components and their functions, Calibration, compensation, and quality control procedures, Hands-on training in instrument startup, operation, and shutdown protocols.

Unit 3

Experimental Design and Multicolor Panel Development, Strategies for designing flow cytometry experiments, Selection of antibodies and fluorochromes for multicolor panels, Controls and standards in flow cytometry assays.

Unit 4

Data Acquisition and Analysis, Techniques for data acquisition and gating strategies, Use of flow cytometry software for data analysis, Interpretation of histograms, dot plots, and statistical outputs.

Unit 5

Cell Sorting Techniques, Principles and mechanics of fluorescence-activated cell sorting (FACS), Sorting strategies: purity vs. yield considerations, Practical sessions on setting up and performing cell sorting experiments.

Unit 6

Applications of Flow Cytometry, Immunophenotyping and analysis of immune cell subsets, Assessment of cell cycle, apoptosis, and proliferation, Applications in stem cell research and cancer immunology.

Objectives and Outcomes

Course Outcome:

CO1: Understand the principles and components of flow cytometry.

CO2: Operate flow cytometers, including calibration and quality control.

CO3: Design flow cytometry experiments with multicolor panels.

CO4: Acquire and analyze data using gating strategies and flow cytometry software.

CO5: Master FACS techniques for sorting cells based on purity and yield.

CO6: Apply flow cytometry in immunophenotyping, cell cycle analysis, apoptosis, and stem cell research.

Program Outcomes (POs):

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

CO–PO Mapping:

COs

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

CO1

3

2

–

–

2

–

–

–

–

–

–

2

CO2

3

–

–

2

3

–

–

–

–

–

–

2

CO3

3

3

3

3

3

–

–

–

–

–

–

2

CO4

2

3

3

3

3

–

–

–

–

–

–

2

CO5

3

2

2

2

3

–

–

–

–

–

–

2

CO6

3

3

3

3

3

2

–

–

–

–

–

2

PROGRAM SPECIFIC OUTCOMES (PSO):

PSO1: Mastery of Nanomaterial Science and Characterization

PSO2: Understanding of Cellular and Molecular Systems

PSO3: Application of Nanobiotech in Diagnostics and Therapy

PSO4: Experimental Design and Data Analysis

PSO5: Research Competency and Methodological Rigor

PSO6: Industry and Career Readiness

CO–PSO Mapping

COs

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

CO1

–

3

–

–

2

2

CO2

–

2

–

2

2

3

CO3

–

3

3

3

3

3

CO4

–

3

3

3

3

3

CO5

–

2

3

2

3

3

CO6

–

3

3

3

3

3

Text Books / References

Textbook:

Ormerod MG. Flow Cytometry: A Practical Approach. 3rd ed. Oxford: Oxford University Press; 2000.

Reference Book:

Robinson JP, Darzynkiewicz Z, Dressler LG, et al., editors. Current Protocols in Cytometry. New York (NY): John Wiley & Sons; 2001.

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