| Course Name | Cell culture & Animal Lab |
| Course Code | 25NBT582 |
| Program | M. Sc. Nanobiotechnology (NBT) |
| Semester | 1 |
| Credits | 2 |
| Campus | Kochi |
General lay out of a cell culture lab, physical environment needed for the cell culture, growth media and its composition, BSC and its use in cell culture and how to work in a BSC, contamination during cell culture and how to control it, culturing and splitting of cell lines, cryopreservation of cells and cell viability assays.
Hands-on cell culture work, media changes, seeding, splitting adherent cells, cell counting, reseeding, and safe disposal
Animal handling techniques, animal feed, gavage, different routes of injection, ethical treatment of animals and Institutional Animal Ethics Committee policies
Pre-requisites: Basic understanding of experimental research
COURSE OUTCOME
CO 1: To understand the general layout of the cell culture laboratory
CO 2: To understand various physical environments needed for cell culture and be familiarized with general cell culture works in BSCs
CO 3: To learn the basic techniques of handling, splitting, seeding, maintaining and preserving cell lines, identify contaminations in cell cultures and to control them
CO 4: To have basic knowledge on handling of laboratory animals including ethical treatment, familiarizing with IAEC policies
CO 5: To have knowledge on animal feed, gavage, different routes of injection and sample collection.
PROGRAM OUTCOME:
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
0 – No affinity; 1 – low affinity; 2 – Medium affinity; 3 – High affinity
CO-PO MAPPING:
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PROGRAM SPECIFIC OUTCOMES (PSO):
PSO1: Mastery of Nanomaterial Science and Characterization
PSO2: Understanding of Cellular and Molecular Systems
PSO3: Application of Nanobiotech in Diagnostics and Therapy
PSO4: Experimental Design and Data Analysis
PSO5: Research Competency and Methodological Rigor
PSO6: Industry and Career Readiness
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Total number of lab sessions: 15
COURSE OUTCOME:
After successful completion of the course, students will be able to:
REFERENCES:
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