Course

Unit 1

Introduction to Systems Biology: Bioinformatics to Systems, Biology of systems, Major and minor components of the systems, Systems Genomics

Unit 2

Experimental methods behind Systems Biology: Top-down and bottom-up systems biology, Analytical and experimental methods in ascertaining protein-protein interactions (PPI)

Unit 3

The essentials of Systems Biology: Metabolic fluxes, Introduction to mathematic models: COPASI models and pathway analysis

Unit 4

Systems Biology Tools: GeneMania, String-db, MINT, Genecards, Phenolyzer, IntAct, BIND, DIP, Biogrid; Visualizers for understanding PPI ? Cytoscape, VisANT and Osprey; Regulatory/Systems Genomics; Mini project, quizzes and group discussions

Unit 5

Pathways and Models: Molecules to Pathways, Pathways to Networks, Networks to Models, examples and case studies.

LEARNING OBJECTIVES:

 The course helps the students to understand the interactions within biological networks and thereby asking some of the fundamental issues concerning protein-protein interactions (PPIs), their need and usage.

COURSE OUTCOMES:

After completing the course, students shall be able to

CO1. Learn & identify protein interactions

CO2. Find the bona fide candidate interacting pairs for their targets of interest.

CO3. Compare the in-silico approaches with well-known validated approaches.

CO4. Learn the essentials of molecular pathways using top-down approaches.

CO5. The course is e interactive and self-contained with rigorous brainstorming.

CO6. There will be easy hands-on exercises using visualizers and command line.

REFERENCES:1. Lu, H., Zhou, Q., He, J. et al. Recent advances in the development of protein?protein interactions modulators: mechanisms and clinical trials. Sig Transduct Target Ther 5, 213 (2020). https://doi.org/10.1038/s41392-020-00315-3 .2. Chuang HY, Hofree M, Ideker T. A decade of systems biology. Annu Rev Cell Dev Biol. 2010; 26:721-44. doi: 10.1146/annurev-cellbio-100109-104122.