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Abstract : In this work, "Poly(9,9-di-noctylfluorene-alt-benzothiadiazole) (F8BT)" is used for the first time in a multilayered SPR sensor. The gold (Au), F8BT, black phosphorous (BP), and urease enzyme layers are added to the BK7 prism to assist high light efficiency, activating surface plasmons, minimizing damping effect, increasing absorption, and urea detection, respectively. The performance of the sensor in the visible wavelength region is examined using the transfer matrix technique (TMM). The optimized thickness of Au and F8BT layers are 45 nm and 10 nm, respectively. Four alternative sensor configurations are examined based on the material layers utilized in the presence of urea-treated urine sample concentrations of 0.625 gm/dl, 1.25 gm/dl, 2.5 gm/dl, 5 gm/dl, and 10 gm/dl. Sensitivity, quality factor, and detection accuracy calculations are done to evaluate each structure's performance. A maximum sensitivity of 240 , QF of 25.92 , and DA of 0.301 are achieved for the Au/F8BT/BP/Urease sensor construction. Additionally, a two-dimensional finite difference time domain (FDTD) method-based tool is used to study the field confinement in the metallic layer. This is a preview of subscription content, log