Syllabus
Theory
Biochemistry – Introduction and importance, Properties of water, pH and buffer, plant cell and its components. Biomolecules – Structure, classification, properties and function of carbohydrates, amino acids, proteins, lipids and nucleic acids. Vitamins – physiological and metabolic role. Enzymes: General properties; Classification; Mechanism of action; Michaelis and Menten and Line Weaver Burk equation and plots; Introduction to allosteric enzymes, use of enzymes. Metabolic energy and its generation – Metabolism – Basic concepts, Glycolysis, Citric acid Cycle, Pentose phosphate pathway, oxidative phosphorylation, Fatty acid oxidation. Biosynthetic Pathways – Photosynthesis, Gluconeogenesis, nitrogen fixation, fatty acid and starch formation. Regulation of metabolic pathways. Secondary metabolites, Terpenoids, Alkaloids, Phenolic and their applications in food and pharmaceutical industries.
Practical
Preparation of standard solutions and reagents, Determination of pH, Qualitative tests of carbohydrates and amino acids, Quantitative estimation of soluble sugars and starch, Estimation of protein by Kjeldhal method and Lowry’s method, Preparation of mineral solution from ash, Estimation of fat by Soxhlet method, Determination of acid value, saponification value and iodine number, Estimation of ascorbic acid, Qualitative/quantitative tests of secondary metabolites.