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Course Detail

Course Name Laboratory Techniques in Translational Research II
Course Code 24TM517
Program M.Sc. in Translational Medicine
Semester II
Credits 2
Campus Faridabad


Unit 1:
Protein Techniques, Protein extraction and purification methods, SDS-PAGE and western blotting, Enzyme-linked immunosorbent assay (ELISA)
Unit 2:
Immunohistochemistry and Immunofluorescence, Tissue fixation and processing, Antibody staining and detection, Microscopy and image analysis
Unit 3:
Flow Cytometry and Cell Sorting, Principles of flow cytometry, Fluorescent labelling and analysis of cells, Cell sorting techniques

Objectives and Outcomes

Laboratory Techniques in Translational Medical Research is a graduate-level course designed to provide students with hands-on experience in the laboratory techniques commonly used in translational medical research. The course will cover essential laboratory skills, experimental design, data analysis, and interpretation relevant to translational research in medicine. Through laboratory sessions, demonstrations, lectures, and practical exercises, students will gain proficiency in various molecular, cellular, and biochemical techniques essential for conducting translational research.

Course outcome

CO1: To acquire proficiency in a variety of laboratory techniques commonly used in translational medical research.
CO2: To understand the principles underlying each laboratory technique, including experimental design, data analysis, and interpretation.
CO3: To learn about the application of laboratory techniques in studying disease mechanisms, biomarker discovery, and therapeutic development.
CO4: To develop critical thinking skills in experimental design, troubleshooting, and result interpretation.
CO5: To gain hands-on experience in laboratory safety practices, equipment operation, and data documentation.
CO6: To apply laboratory techniques to address specific research questions relevant to translational medicine.

Program outcome (PO)

PO1: Utilize scientific principles and methodologies to design innovative solutions for data analysis, experimentation, and product development for challenges in translational research.
PO2: Recognize the importance of environmental sustainability in translational research and strive to minimize adverse environmental impacts.
PO3: Engage in ethical conduct, leadership, active listening, constructive feedback, and interpersonal communication to facilitate productive collaborations and knowledge exchange.
PO4: Acquire fundamental and advanced knowledge and skills in project management, financial planning, and entrepreneurship relevant to translational research ventures and initiatives.
3 = High Affinity, 2 = Medium Affinity, 1 = Low Affinity, – = No Affinity

CO 1 3 1 3
CO 2 3 1 3
CO 3 3 1 3
CO 4 3 1 3
CO 5 3 3 3 3
CO 6 3 1 3

Program Specific Outcome (PSO)

PSO1: Addresses the complexity of interdisciplinary sciences in biological and medical contexts.
PSO2: Deals with regulatory affairs in medicine, covering topics such as ethical considerations and regulatory frameworks.
PSO3: Covers compounds as drugs and their efficacy, involving pharmacology and drug development.
PSO4: Explores the intersection of bioinformatics and artificial intelligence in biology and medicine.
PSO5: Deals with technology in personalizing medicine, involving precision medicine approaches.
PSO6: Focuses on communicating and disseminating science and medicine to the public, involving science communication and public outreach efforts.

CO 1 3 3 3 3 3 2
CO 2 3 3 2 3 1 2
CO 3 3 3 3 2 2 2
CO 4 3 3 1 1 2 2
CO 5 3 3 1 1 2
CO 6 3 2 2 2 1


Alberts, B., 2017. Molecular biology of the cell. (5th Edition) Garland science.
Lodish, H.F., 2008. Molecular cell biology. (7th Edition) Macmillan.

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