Pre-requisites: Basic understanding of biology and biochemistry and immunology
Total number of classes:30
Preamble:
Students will be familiarized with the basics as well as advanced methods in microbiology and immunology that can be used for disease diagnosis. Provide hands-on training for isolation of microbes in pure culture and antibiotic susceptibility assays. Provide the students with practical skills on lymphocyte isolation and analysis.
Practicals:
Experiment 1: Observation & Identification of Immune Cells,
Experiment 2: Observation and identification of the structure of lymphoid organs,
Experiment 3: Blood grouping,
Experiment 4: Separation of Mononuclear cells using density gradient centrifugation,
Experiment 5: Isolation of Monocytes from the Mononuclear Cells,
Experiment 6: Induction of Monocytes to M1 and M2 Macrophages,
Experiment 7: Evaluation of inflammatory and anti-inflammatory cytokines produced by M1 and M2 macrophages through ELISA.
Experiment 8: Bacterial and fungal culture medium preparation,
Experiment 9: Pure culture technique (eg: streaking and sub culturing),
Experiment 10: Gram staining, Fungal staining, Motility assay
Bacterial growth curve by measuring turbidity and viable count
Experiment 11: Antibiotic sensitivity assay; MIC and MBC determination,
Experiment 12: Biofilm assay, MBIC and MBEC determination,
Experiment 13: Phage titration assay