Unit 1
Unit 1 (Lectures 10)
DNA: Structure and function, Chromosome and chromatin, Genetic code, wobble hypothesis, RNA and types of RNA (rRNA, snRNA, siRNA, snoRNA), Proteins and their structure
| Course Name | Molecular Biology |
| Course Code | 25CLG501 |
| Program | M. Sc. Clinical Genomics |
| Semester | 1 |
| Credits | 3 |
| Campus | Kochi |
Unit 1 (Lectures 10)
DNA: Structure and function, Chromosome and chromatin, Genetic code, wobble hypothesis, RNA and types of RNA (rRNA, snRNA, siRNA, snoRNA), Proteins and their structure
Unit 2 (Lectures 10)
DNA replication and its regulation, Homologous and site-specific recombination, DNA repair
Unit 3 (Lectures 10)
Transcription and its regulation, Translation and its regulation, Gene structure, Repeats and clusters, Gene expression regulations: operon, Epigenetics
Unit 4 (Lectures 5)
Types of mutations, Genetic system of mitochondria
Unit 5 (Lectures 10)
Gene identification, promoter identification, Molecular biology techniques: Isolation and Quantification of DNA/RNA, PCR, Reverse transcriptase PCR, Real Time PCR, DNA Sequence analysis, hybridization (southern, northern and western) and Sanger sequencing.
Pre-requisites: Undergraduate level basic DNA biology
Total number of classes: 45
Preamble
This course will provide a thorough understanding about the biology of DNA and how its regulations are brought about, its functional significance and how it is very much involved in the biochemistry and physiology of the cell.
Course Outcome
CO1. Describe the structure, function, and properties of nucleic acids and proteins as molecular components of the cell.
CO2. Explain the mechanisms and regulation of DNA replication, transcription, and translation in prokaryotes and eukaryotes.
CO3. Analyze the processes of mutation, DNA repair, recombination, and gene regulation, and their relevance in maintaining genomic integrity.
CO4. Interpret the applications of molecular biology techniques in research and clinical diagnostics, including PCR, electrophoresis, blotting, and sequencing.
CO5. Relate molecular biology concepts to clinical genomics, especially in the context of genetic disorders and precision medicine.
Program Outcomes (POs)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
CO – PO Mapping Table:
| C | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| C O | ||||||||||||
| CO 1 | 3 | 1 | – | – | – | – | – | – | – | – | – | 2 |
| CO 2 | 3 | 1 | – | – | 1 | – | – | – | – | – | – | 1 |
| CO 3 | 3 | 2 | 1 | 2 | 2 | 1 | – | – | – | – | – | 1 |
| CO 4 | 3 | 3 | 1 | 2 | 2 | – | – | – | – | – | – | 2 |
| CO 5 | 3 | 1 | 2 | 1 | 3 | 2 | – | – | – | – | – | 3 |
Program Specific Outcomes (PSO):
PSO1. Apply fundamental molecular biology principles to interpret clinical genomic data.
PSO2. Use molecular techniques (e.g., PCR, RT-PCR, sequencing) to detect genetic mutations and biomarkers.
PSO3. Analyze genotype-phenotype correlations in inherited and acquired disorders.
PSO4. Identify pathogenic variants from NGS data and interpret their clinical relevance.
PSO5. Correlate molecular pathways with disease mechanisms and therapeutic targets.
PSO6. Develop and validate diagnostic assays based on molecular biology principles.
PSO7. Utilize molecular biology to support pharmacogenomic profiling and therapy optimization.
PSO8. Integrate multi-omic data (genomic, transcriptomic, epigenomic) for personalized health solutions.
PSO9. Apply molecular knowledge to cancer genomics, infectious diseases, and rare genetic disorders.
PSO10. Translate molecular discoveries into clinical interventions through evidence-based practice.
CO–PSO Mapping Table:
| COs | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 |
| CO1 | 3 | 1 | – | – | – | – | – | – | – | 2 |
| CO2 | 3 | 1 | – | – | 1 | – | – | – | – | 1 |
| CO3 | 3 | 2 | 1 | 2 | 2 | 1 | – | – | – | 1 |
| CO4 | 3 | 3 | 1 | 2 | 2 | – | – | – | – | 2 |
| CO5 | 3 | 1 | 2 | 1 | 3 | 2 | 2 | – | – | 1 |
Evaluation Pattern: 50+50 = 100
| Internal Assessment – 50% | ||
| Periodical 1 | Exam | 20% |
| Periodical 2 | Exam | 20% |
| Continuous Assessment | Assignment/ Test/ Quiz | 10% |
| 50% | ||
| End Semester Examination- 50% | ||
| Theory Exam | 50% | |
| 50% | ||
| Total | 100% | |
Text Book:
Lewin’s Essential Genes, Jocelyn E. Krebs, Benjamin Lewin, Elliott S. Goldstein, Stephen T. Kilpatrick, Jones& Bartlett Publishers, 2013 – Science – 847 pages
References:
Molecular Biology of the Gene, Seventh Edition, James D. Watson, Cold Spring Harbor Laboratory; Tania A. Baker, Massachusetts Institute of Technology; Alexander Gann, Cold Spring Harbor Laboratory; Michael Levine, University of California, Berkeley; Richard Losick, Harvard University, 2013
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