Back close

Ribotyping of Fish Microflora

Start Date: Tuesday, Jan 01,2013

End Date: Thursday, May 30,2013

School: School of Biotechnology

Thematic Area: Biotech

Project Incharge:Ajith Madhavan
Co-Project Incharge:AncyThaha,Jithin James , SharonD’Morris , SruthiChandran , Vishnu U L
Ribotyping of Fish Microflora

Microflora of fish intestines appear to vary with the complexity of the fish digestive system. The genera present in the gut generally seem to be those from the environment or diet which can survive and multiply in the intestinal tract. The main aim of the work is to identify the organism present in the gut of fish through ribotyping technique. To start up with the procedure first standardized the ribotyping technique. For standardization of the procedure a gram+ve(Bacillus) and a gram-ve (E.coli) was used. Genomic DNA was successfully isolated from both organisms. Hot shot method was performed. PCR method was standardized. As the procedure standardized the microflora present in the fish gut was successfully isolated by serial dilution. Sixteen morphologically different colonies were got. DNA was isolated using hotshot method. Extracted DNA was subjected to PCR amplification. Four samples showed strong bands and send for sequencing.

Related Projects

Benchtop Nanoscale patterning using soft lithography for the printing of DNA molecules
Benchtop Nanoscale patterning using soft lithography for the printing of DNA molecules
Construction of a Prototype of Microbial Fuel Cell
Construction of a Prototype of Microbial Fuel Cell
Biofibre from Agricultural Wastes: Bio Bleaching of Banana Fibre using Microbial Enzymes
Biofibre from Agricultural Wastes: Bio Bleaching of Banana Fibre using Microbial Enzymes
Affordable paper based microfluidics point of care testing device for liver function
Affordable paper based microfluidics point of care testing device for liver function
Bioapproach: Synthesis of Silver Nanoparticles from Mangrove Endophytes
Bioapproach: Synthesis of Silver Nanoparticles from Mangrove Endophytes
Admissions Apply Now