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Combinatorial Effect of D-amino Acids and Tetracycline against Pseudomonas Aeruginosa Biofilm

Publication Type : Journal Article

Thematic Areas : Biotech

Publisher : International Journal of Pharmacy and Pharmaceutical Sciences

Source : International Journal of Pharmacy and Pharmaceutical Sciences, Volume 8, Issue 11, p.216-220. (2016)

Url : https://innovareacademics.in/journals/index.php/ijpps/article/view/9531

Keywords : biofilm, D-amino acid, P aeruginosa, tetracycline

Campus : Amritapuri

School : School of Biotechnology

Center : Biotechnology, Cell Biology

Department : biotechnology

Year : 2016

Abstract : Objective: The present study attempted to evaluate the anti-biofilm activity of D-amino acids (D-AAs) on Pseudomonas aeruginosa and determine if the combination of D-AAs with tetracycline enhances the anti-biofilm activity in vitro and ex vivo. Methods: Different D-AAs were tested for antibiofilm activity against wild type P. aeruginosa PAO1 and two multidrug resistant P. aeruginosa clinical strains in the presence of sub inhibitory concentrations of tetracycline using crystal violet microtitre plate assay. Results were further validated using in vitro wound dressing and ex vivo porcine skin models followed by cytotoxicity and hemocompatibility studies. Results : D-tryptophan (5 mmol) showed 61 % reduction in biofilm formation of P. aeruginosa . Interestingly combinatorial effect of 5 mmol D-tryptophan and 0.5 minimum inhibitory concentration (MIC) (7.5µg/ml) tetracycline showed 90% reduction in biofilm formation. 5 mmol D-methionine shows 28 % reduction and combination with tetracycline shows 41% reduction in biofilm formation of P. aeruginosa . D-leucine and D-tyrosine alone or in combination with tetracycline did not show significant anti-biofilm activity. D tryptophan-tetracycline combination could reduce 80 % and 77 % reduction in biofilm formation in two multi drug resistant P. aeruginosa clinical strains. D-tryptophan-tetracycline-combination could also reduce 76% and 66% reduction in biofilm formation in wound dressing model and porcine skin explant respectively. The cytotoxicity and hemocompatibility studies did not show significant toxicity when this combination was used. Conclusion: The results established the potential therapeutic application of D-tryptophan alone or in combination with tetracycline for treating biofilm associated clinical problems caused by P. aeruginosa

Cite this Research Publication : Jayalekshmi H., Norin Mary G Victus, R. Anoop, T. M. Sarath, Sajin Sali, C. .Harikrishnan, N. Kaushik, Dr. Geetha Kumar, and Dr. Bipin G. Nair, “Combinatorial effect of d-amino acids and tetracycline against pseudomonas aeruginosa biofilm”, International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 11, pp. 216-220., 2016.

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