Back close

Fabrication of Paper Microfluidics POCT Device for the Colorimetric Assay of Alkaline Phosphatase

Publication Type : Conference Paper

Thematic Areas : Biotech

Publisher : IEEE Indicon

Source : IEEE Indicon 2018 (Scopus)

Url :

Campus : Coimbatore

School : School of Engineering

Center : Biosensor Research Lab

Department : Electronics and Communication, Sciences

Year : 2018

Abstract : Alkaline phosphatase (ALP) serves as an important biomarker for the diagnosis of various diseases including liver malfunction, bone diseases, breast and prostate cancers, and diabetes. This paper discusses the fabrication and testing of a paper-based Point-of-care testing (POCT) device for the colorimetric detection of alkaline phosphatase (ALP). The device was fabricated using a commercial wax printer followed by reagent casting on the patterned paper. In the presence of ALP concentration of 30 - 500 U/L, the reagent paranitrophenylphosphate (PNPP) changes to yellow color with increasing intensity. The different color intensities observed for different ALP concentrations were captured and a color band was developed for the visual interpretation. The RGB and HSV of the captured color intensities were processed using MATLAB to obtain the corresponding ALP concentration.

Cite this Research Publication : Reshmi PE, John S, Sachin Kumar S, Ramachandran T, Satheesh Babu TG, Fabrication of Paper Microfluidics POCT device for the colorimetric assay of Alkaline phosphatase, IEEE Indicon 2018 (Scopus)

Admissions Apply Now