Publication

Abstract : Background Dry Eye (DE) is a condition marked by tear film instability and chronic inflammation, leading to visual impairment. Conventional artificial tears often contain preservatives that exacerbate DE and fail to address underlying tissue damage. In Ayurveda, DE symptoms corelates with S ushkakshipaka, managed by normalizing vata pitta derangement. This study evaluates the mechanism of Ghrita Manda Ashchyotana in a Benzalkonium Chloride (BAC)-induced murine dry eye model. Methodology 12 male Swiss albino mice (24 eyes) were divided into: saline-treated controls (3 mice), and dry eye-induced mice treated with either Ghrita Manda (Trial), preservative-free eye drops (Positive control), or left untreated (Negative control). Assessments included Tear Film Break-Up Time (TFBUT), fluorescein scores, corneal Hematoxylin and Eosin (H&E) staining, and IL-6 expression after 14 days. Results Ghrita Manda significantly improved TFBUT and reduced fluorescein scores compared to the negative control (p < 0.001). Corneal integrity was preserved without hyperplasia in the trial group. A notable reduction in IL-6 expression was observed compared to the positive control. Conclusion Ghrita Manda demonstrates potential as an effective DE therapy, offering lubrication, corneal protection, and anti-inflammatory benefits, aligning with Ayurvedic principles for managing vata pitta derangements of the ocular surface.