Summary
‘Instrumental Methods of Analysis – Practical’ is a course offered in the seventh semester of B. Pharm program at School of Pharmacy, Health Sciences campus, Amrita Vishwa Vidyapeetham.
Course Content
Course Duration: 4 Hours/Week
- Determination of absorption maxima and effect of solvents on absorption maxima of organic compounds
- Estimation of dextrose by colorimetry
- Estimation of sulfanilamide by colorimetry
- Simultaneous estimation of ibuprofen and paracetamol by UV spectroscopy
- Assay of paracetamol by UV- Spectrophotometry
- Estimation of quinine sulfate by fluorimetry
- Study of quenching of fluorescence
- Determination of sodium by flame photometry
- Determination of potassium by flame photometry
- Determination of chlorides and sulphates by nephelo turbidometry
- Separation of amino acids by paper chromatography
- Separation of sugars by thin layer chromatography
- Separation of plant pigments by column chromatography
- Demonstration experiment on HPLC
- Demonstration experiment on Gas Chromatography
Reference
Recommended Books (Latest Editions)
- Instrumental Methods of Chemical Analysis by B.K Sharma
- Organic spectroscopy by Y.R Sharma
- Text book of Pharmaceutical Analysis by Kenneth A. Connors
- Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
- Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
- Organic Chemistry by I. L. Finar
- Organic spectroscopy by William Kemp
- Quantitative Analysis of Drugs by D. C. Garrett
- Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi
- Spectrophotometric identification of Organic Compounds by Silverstein