Course

Unit 1

Historical Account: Discovery of DNA as genetic material, Griffith’s experiment, Hershy and Chase warring blender experiment, Chargaff’s rule

Unit 2

Macromolecular Description: Structure of DNA, RNA and Protein Basic mechanism of replication.

Unit 3

Flow Of Information-Central Dogma: Basic mechanism of replication, transcription, translation.

Unit 4

Regulation In Prokaryotes and Eukaryotes: Gene regulation in prokaryotes and eukaryotes, positive regulation, negative regulation, attenuation, gene regulation in lambda phage life cycle, RNA processing and post transcriptional regulation.

Unit 5

Regulatory Mechanisms: Eukaryotic transcription factors, enhancers, silencers, insulators, chromatin structure and gene regulation, Translational regulation in prokaryote and eukaryotes, Post translational modification and protein stability.

LEARNING OBJECTIVES:

Introducing and strengthening the basic molecular processes that are common to all living organisms. This course will form the pillar of knowledge which in turn help the students for better understanding of various other subjects in the field of biotechnology.

COURSE OUTCOMES: 

After completing the course, students shall be able to

CO1. Learn and understand the important discoveries that are made in the field of molecular biology.

CO2. Understand the detailed structure of the double helical nature of DNA as proposed by scientists like Watson and Crick. 

CO3. To learn different levels of organizations that regulate the condensation of DNA that leads to the compact metaphase chromosome. 

CO4. To learn key molecular events that occur during the transcription and translation processes that leads the protein synthesis from specific genes. 

CO5. Understanding the mechanisms that regulate the regulation of gene expression in both prokaryotes and eukaryotes.

CO6. Learn about the molecular events that happen during the replication of DNA prior to cell division. 

2007. Molecular Biology of the gene, James D Watson et al, 7^TH Edition, Pearson, 2007.
2008. Gene VIII, Benjamin Lewin, 8^th edition, Pearson publishers, 2003.
2009. Molecular biology, David Freifielder, 2^nd edition, Barlett and Jones, 1986.