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Course Detail

Course Name Cell and Molecular Biology Lab
Course Code 25BIO282
Program B.Sc. (Hons.) Microbiology
Semester 3
Credits 2
Campus Amritapuri

Syllabus

  1. Micro pipetting.
  2. Lignin staining: comparison between monocots and dicots.
  3. Plant and animal cell identification.
  4. Mitosis in onion root tip.
  5. Genomic DNA isolation by CTAB method from different sources like leaf, flowers and fruits of plants.
  6. Spectrophotometry
  7. Agarose gel electrophoresis.
  8. Polyacrylamide gel electrophoresis

Objectives and Outcomes

LEARNING OBJECTIVES:

Hands-on experience of research in Cell Biology.  Focuses on using microscopy to investigate various structural features of cells as well as understanding the state of the cells (resting/dividing). Lab also focuses on basic molecular biology techniques including DNA isolation and electrophoresis.

COURSE OUTCOMES:

After completing the course, students shall be able to

CO1: Illustrate microscopic techniques to differentiate between plant and animal cells

CO2: Illustrate various stages of mitosis.

CO3: Perform genomic DNA isolation and characterisation.

CO4: Perform protein separation techniques

Text Books / References

REFERENCES:

  1. Cell and Molecular Biology: Concepts and Experiments – Gerald Karp, 3rd Edition, 2006.
  2. Cell and Molecular Biology: A lab manual -K.V. Chaitanya, 2013.

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