Course

1. Isolation and identification of normal skin flora.
2. Preparation of blood agar and demonstration of hemolysis.
3. Staining: Acid fast staining , Negative staining.
4. Isolation and identification of unknown bacteria from pure culture.
5. Isolation and identification of unknown bacteria from mixed culture.
6. Antibiotic sensitivity test.
7. Demonstration of WIDAL Test

LEARNING OBJECTIVES:

Learn about handling of pathogens, common diagnostics methods like staining, culture techniques, antibiotic sensitivity, and identification of pathogens.

 COURSE OUTCOMES:

After completing the course, students shall be able to

CO1. Understand various methods of screening industrially important microorganisms from different sources. 

CO2. Learn the technique of immobilization of cells like yeast.

CO3. Demonstrate the ability of microorganisms for nitrogen fixation and phosphate solubilization.

COURSE OUTCOMES:

After completing the course, students shall be able to  

CO1. This course describes how to make decisions about the pathogenicity of organisms associated with human infections.

CO2. Explain how to apply appropriate microbiology laboratory techniques, methodologies, instruments and equipment in accordance with current laboratory safety protocol.

CO3. Provide the opportunity to practise in microbiology laboratory techniques and to draw and report appropriate conclusions from the analysis of experimental data.

REFERENCES:

1. Microbiology-A laboratory Manual-James G.Cappucino,Natalie Sherman. 10^th Edition 2014.
2. Color Atlas of Medical bacteriology-Luis M de la Maza,Marie T Pezzlo 3^rd Edition 2020.