Course

1. Isolation and screening of antibiotic producers by crowded plate technique.2. Isolation of Actinomycetes from soil, Secondary screening protocols-Giant colony technique, Secondary screening protocols-Kirby-Bauer method.3. Isolation and screening of microorganism producing proteases.4. Isolation and screening of microorganisms producing amylases.5. Isolation of Nitrogen fixers from soil.6. Isolation of phosphate solubilizers from soil.7. Immobilization of yeast in alginate beads for ethanol production.8. Production of citric acid.

LEARNING OBJECTIVES:

To provide hands on experience on isolating and evaluating the industrial potential of microorganisms from various sources. This course helps students to work with small scale fermenters and learn their basic working principles.

COURSE OUTCOMES:

After completing the course, students shall be able to

CO1. Understand various methods of screening industrially important microorganisms from different sources.

CO2. Learn the technique of immobilization of cells like yeast.

CO3. Demonstrate the ability of microorganisms for nitrogen fixation and phosphate solubilization.

REFERENCES:1. James G. Cappuccino and Natalie Sherman Microbiology: A Laboratory Manual,10th edition, 2014.2. Arnold L Demain , Julian E Davies, Manual of Industrial Microbiology and Biotechnology, 2nd edition, 1996.