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Course Detail

Course Name Biochemistry Lab
Course Code 25BIO181
Program B.Sc. (Hons.) Microbiology
Semester 2
Credits 2
Campus Amritapuri

Syllabus

SYLLABUS:

  1. Preparation of Laboratory Solutions and Buffers.
  2. Verification of Beer-lamberts Law using Potassium Dichromate.
  3. Estimation of Amino acids by Ninhydrin Method.
  4. Separation of Amino acids using TLC.
  5. Isoelectric Precipitation of Casein from Milk.
  6. Qualitative Analysis of Carbohydrates.
  7. Qualitative Analysis of Amino acids.
  8. Estimation of Reducing Sugar using DNS Method.

Objectives and Outcomes

LEARNING OBJECTIVE:

This course deals with basic biochemical calculations and preparations of various reagents, qualitative and quantitative analysis of both carbohydrates and amino acids, and chromatography techniques.

COURSE OUTCOMES:

After completing the course, students shall be able to

CO1: Explain buffer and reagent preparations

CO2: Operate basic laboratory biochemical equipment.

CO3: Perform qualitative and quantitative analysis of biomolecules.

Text Books / References

REFERENCES:

  1. “Experimental Biochemistry”, Beedu Sashidhar rao, Vijay Deshpande, I K International Pvt. , ISBN 81-88237-41-8 1st edition 2005.
  2. Laboratory Manual in Biochemistry; J.Jayaraman, New Age International Private Limited, Second edition 2011.

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