Course

Syllabus

Theory

Introduction, History, Advantages and limitations. Types of cultures (seed, embryo, organ, callus, cell); Stages of micro propagation; Axillary bud proliferation (Shoot tip and meristem culture, bud culture); Organogenesis (callus and direct organ formation); Somatic embryogenesis; Cell suspension cultures; production of secondary metabolites; Somaclonal variation; Cryopreservation.

Practical

Identification and use of equipment in tissue culture Laboratory; Nutrition media composition; Sterilization techniques for media, containers and small instruments; Sterilization techniques for explants; Preparation of stocks and working solution; Preparation of working medium; Culturing of explants: Seeds, shoot tip and single node; Callus induction; Induction of somatic embryos regeneration of whole plants from different explants; Hardening procedures

Objective

1. To educate the students about the sterilization techniques for explants, preparation of stocks and working solution, culturing of explants, regeneration of whole plants from different explants and hardening procedures.

Course Outcome:

Any student who has undergone the course shall be able to:

Suggested Readings

1. ICAR. 2001. Handbook of Horticulture. ICAR.
2. Jha Timir Baran and Ghosh Biswajit. 2016. Plant Tissue Culture: Basic and Applied.
3. (2nd Edn.). Platinum Publishers, 439p.
4. Kumar N. 2020. Introduction to Horticulture. Oxford & IBH Publishing Company Pvt Ltd.
5. Singh Jitendra. 2008. Basics of Horticulture. Kalyani Publishers.