Course

Unit I

Amino acids, Proteins and PeptidesClassification, Stereochemical aspects, physical properties, Ionic properties, spectral properties, essential and non-essential amino acids, chemical reactions of amino acids, Industrial preparation and chemical synthesis of amino acids. Ionic properties of proteins, protein structure, protein purification, protein structure determination, proteomics and protein function, solid phase peptide synthesis, biologically important peptides.

Unit II

EnzymesIntroduction to Enzymes, Classification of enzymes, mechanism of enzyme action, immobilized enzymes and enzyme technology, enzyme- analogue built polymers, design of molecular clefts, enzymes in synthetic organic chemistry. Enzymes in biological systems

Unit III

Molecular Biology and Bioinformatics
Structure of nucleic acids, genes and genome complexity, functions of nucleic acids, isolation and separation of nucleic acids, molecular analysis of nucleic acid sequences, nucleotide sequencing of DNA.

Unit IV

Immunochemical Techniques
Production of antibodies, purification and fragmentation of immunoglobulins, immunoprecipitation, labelling antibodies, immunoblotting, immunoassays, immunohistochemistry/cytochemistry.

Unit V

Recombinant DNA and Genetic Analysis
Constructing gene libraries, cloning vectors, hybridization and gene probes, application of gene cloning, expression of foreign genes, pharmacogenomics.

• CO1: Explain the structural, setereochemical and physicochemical properties of amino acids, perptides and proteins and their biological significance.
• CO2: Describe enzyme classification, mechanism of action and role of enzymes in organic synthesis and biology.
• CO3: Explain nuclei acid structure, genome organization and DNA sequencing for molecular analysis of genetic information.  
• CO4: Describe immunochemical techniques including antibody production, labeling and immune assays.
• CO5: Explain recombinant DNA techniques and genetic analysis methods and their applications in gene expression, biotechnology and pharmacogenomics.

References

1. Hermann Dugas, ‘Bioorganic Chemistry – A Chemical Approach to Enzyme Action’, 3^rd edition, Springer.
2. Keith Wilson and John Walker, ‘Principles and Techniques of Biochemistry and Molecular Biology’, 6^th edition, Cambridge University Press.