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Course Detail

Course Name Recombinant DNA Technology Lab
Program M. Sc. in Biotechnology, M. Sc. in Microbiology
Semester Two
Credits Two
Year Taught 2019


Transformation methods, genomic DNA isolationPlasmid DNA isolationrestriction digestion of Plasmid and genomic DNA, elution of DNA by low melting gel agaroseLigation, insert analysis, isolation of RNA, northern blotting, PCR, RT-PCR, Recombinant protein expression, purification and refolding.

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