Qualification: 
Ph.D, M. Pharm.
Email: 
leenakpappachen@aims.amrita.edu

Dr. Leena K. Pappachan joined Amrita as Senior Lecturer in November 2007. She completed Bachelor’s degree from K.M. College of Pharmacy, Madurai & Master’s degree in Pharmaceutical Chemistry from Dr. M.G.R. Medical University. She completed doctorate degree from Karpagam University in 2014. She has 20 national and international publications to her credit. She has been a project guide for both M. Pharm and B. Pharm students. Her current areas of interest include phytochemical screening, drug design and synthesis of medical compounds.

Publications

Publication Type: Journal Article

Year of Publication Publication Type Title

2017

Journal Article

Dr. Leena K. Pappachen, Dr. Subin Mary Zachariah, and Chandran, D., “In silico design, synthesis and characterization of some novel benzothiazole derivatives as anticancer agents”, Asian Journal of Pharmaceutical and Clinical Research, vol. 10, pp. 150-155, 2017.[Abstract]


Objectives: Cancer is a disease characterized by uncontrollable, irreversible, independent, autonomous, uncoordinated, relatively unlimited, and abnormal overgrowth of tissues. Breast cancer is the second most common type of cancer after lung cancer. The aim of the study is to carry out the docking studies, synthesis, and antitumor activities of benzothiazole derivatives containing oxadiazole groups or amino groups. Methods: The docking studies of benzothiazole derivatives were done with known anticancer targets like estrogen receptor using Argus lab and AutoDock programs and compared with the standard drug tamoxifen. Based on the results obtained from the molecular modeling studies, the derivatives were selected for the synthesis. The synthesized compounds were characterized by melting point, thin layer chromatography, InfraRed,1H NMR,13CNMR, mass spectral data, and screened for their in-vitro anticancer activities. Results: The docking scores obtained for benzothiazole derivatives (BT1, BT2, BT3, BT4) and std. tamoxifen from the preliminary docking program using Argus Lab were -9.68, -9.4, -9.59, -11.1988, -9.71 and using AutoDock program were -6.29, -5.25, -7.19, -7.48, -3.86, respectively. All the four derivatives were synthesized, characterized, and subjected to in vitro anticancer screening by MTT assay in breast cancer (MCF-7) cell lines. Compounds DBT1, DBT2, and DBT3 were the most active compounds against MCF-7 cell lines with inhibitory concentration 50% of 70.0, 64.0 and 65.0, respectively. Conclusion: All the four derivatives show good docking scores when compared to standard drug tamoxifen and can be concluded that all the synthesized benzothiazole ligands show good anticancer property. © 2017 The Authors.

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2017

Journal Article

N. Rajiv, Sreelakshmi, N., Rajan, J., and Dr. Leena K. Pappachen, “A review on synthesis of benzothiazine analogues”, Research Journal of Pharmacy and Technology, vol. 10, pp. 1791-1797, 2017.[Abstract]


Scientific advances mainly involves the development of new and highly reactive compounds. Nitrogen and sulphur containing benzothiazines forms highly active heterocyclic agents. Benzothiazines are prominent class of heterocyclic compound. Many studies have been done in the development of benzothiazine analogues. Microwave assisted synthesis, regioselective techniques and other methods are also used for benzothiazine synthesis. Various methods have been developed for the synthesis of benzothiazine derivatives. Benzothiazines exhibits numerous therapeutic activities like anti-HIV, calcium antagonistic, anti-serotinin, analgesic, anti-mycobacterial anti-microbial, anti-oxidant, anti-rheumatic, anti-inflammatory, anti-hypertensive ,calcium channel blocker, anti-malarial, potassium channel opener, cardiovascular, anthelmintic, neuroprotective, aldose reductase inhibitor, herbicidal, pesticidal etc. This has raised the necessity to explore novel benzothiazine derivatives which can significantly contribute to the development of therapeutically active agents. And the newer development of novel benzothiazines derivatives also involves the same characteristic features and also contribute various activities. © RJPT. All rights reserved.

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2017

Journal Article

Dr. Leena K. Pappachen, Sreedhar, R., Unnikrishnan, A., and James, S., “Pharmacological Activities Of 1, 3, 4-Oxadiazole Derivatives”, . Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 8, no. 2, 2017.

2016

Journal Article

Dr. Leena K. Pappachen, “Synthesis of Oxadiazole Derivatives: An Overview”, International journal of pharmaceutical sciences review and research, vol. 41, no. 2, pp. 142-153., 2016.

2014

Journal Article

D. Chandran, Dr. Leena K. Pappachen, Prathap, M., Jinsha, M. J., and Jilsha, G., “Insilico drug design and molecular docking studies of some novel benzothiazole derivatives as anti-cancer and anti-inflammatory agents”, International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, pp. 203-208, 2014.[Abstract]


Objective: Cancer is a disease characterized by uncontrollable, irreversible, independent, autonomous, uncoordinated and relatively unlimited and abnormal over growth of tissues. Benzothiazole is an important class of heterocyclic compound which possess interesting biological activities like anti-tumor, anti-microbial, anti-tubercular, anti-malarial, anti-convulsant, anthelmintic, analgesic and anti-inflammatory activity. The drugs containing oxadiazole groups were the first effective chemotherapeutic agents which were systematically proved for the prevention and cure of bacterial infection in human beings. The Objective of the study is too carried out the docking studies of benzothiazole derivatives containing oxadiazole groups or amino groups with known anti-cancer and anti-inflammatory targets like estrogen receptor, cox1 and cox2 by using Argus lab and Auto dock programmes. Methods: Docking studies were carried out using Argus lab and Auto dock version 4.0 for all ten ligands and docking scores were compared with the scores of standard drugs Tamoxifen and Indomethacin. Validation of ligands was carried out by using Lipinski rule of five. Results: 10 ligands show higher docking scores and shows better drug-likeness properties as compared to the reference drugs. The compounds show lowest docking energy and hydrogen bondings stabilize the interactions. Conclusion: The study concluded that all benzothiazole derivatives will be significant lead for further investigation of anti-cancer and anti-inflammatory agents. More »»

2013

Journal Article

Dr. Leena K. Pappachen and CHACKO, A. N. N. A. M., “Isolation and Characterization of Flavone Glycoside Vitexin from Peperomia Pellucida Linn”, Journal of Drug Delivery and Therapeutics, vol. 1, pp. 91–92, 2013.[Abstract]


Peperomia Pellucida Linn, widely used in traditional medicine belongs to the family Piperaceae. It has been used for treating abdominal pain, acne ,boils, colic, fatigue, gout, headache, renal disorders, and rheumatic joint pain. The whole plant after collection, authentication and drying, was extracted with petroleum ether, chloroform, methanol and water using soxhlet extractor. The aim of the study was to isolate the phytoconstituents from the methanolic extract of peperomia pellucida by Preparative-Thin Layer Chromatography (P-TLC) and characterized by spectroscopic techniques (UV-visible spectroscopy, infrared spectroscopy,1H and 13C NMR spectroscopy and mass spectrometry). A flavonoidal structure was isolated from the methanolic extract of the plant. The light yellow coloured compound which solubilizes in methanol, ethanol and water having m.p. 203-204Oc and Rf value 0.61 in mobile phase acetonitrile : water: acetic acid (6:4:0.1) was identified by the various spectroscopical methods. So from this study, it is concluded that the isolated compound may be vitexin which is responsible for various pharmacological activities of the plants.

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2013

Journal Article

Dr. Leena K. Pappachen and CHACKO, A. N. N. A. M., “Preliminary Phytochemical Screening and In-vitro Cytotoxicity Activity of Peperomia Pellucida Linn.”, Pharmacie Globale, vol. 4, p. 1, 2013.[Abstract]


Cancer is the major public difficulty and it is one of the top causes of death in the prosperous countries. In spite of much progress in the treatment of cancer by modern systems of medicines and therapy using synthetic drugs, search for newer natural drugs continues because of several complications like cell injury, bone marrow depression, impaired growth, sterility and hair loss that are associated with the prevalent modern cancer chemotherapy. Peperomia pellucida Linn. is widely used in the traditional medicine, belongs to the family Piperaceae. The present study involves in the investigation of various extracts of Peperomia Pellucida for its phytochemical constituents and the cytotoxic potential of P Pellucida. Qualitative phytochemical analysis of the plant extracts were done and all the extracts were screened for in vitro cytotoxic activity by MTT assay method using HEK 293 (Human Epithelial Kidney cell line), HeLa (Human cervical cancer cell line) and HePG2 (Human hepatic carcinoma cell line).It was found that the presence of phytochemicals were maximum in the methanol extract of P Pellucida when compared to other extracts and the methanol extract showed significant cytotoxic activity than all the other extracts against the above mentioned cancer cell lines while was found to be less cytotoxic against normal human kidney cells(HEK 293) displaying safety for normal cells.

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2013

Journal Article

A. Chacko and Dr. Leena K. Pappachen, “In-vitro antioxidant activity and determination of total phenolic, flavonoid contents of Peperomia pellucida linn”, American Journal of Pharmacy & Health Research, vol. 1, no. 7, pp. 93-101., 2013.

2013

Journal Article

Dr. Leena K. Pappachen, G;, A. Nair, Jyothylekshmy, V., and , “Study of Prescribing Pattern of Drugs in the Management of Gestational Diabetes Mellitus”, IJHP, vol. 50, no. 2, pp. 36 - 38, 2013.

2012

Journal Article

E. Varghese, Dr. Leena K. Pappachen, and Dr. S. Sathianarayanan, “Isolation and evaluation of antimicrobial properties of isolated phytoconstituents of fruits of Helicteres isora Linn.”, Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 3, pp. 959-964, 2012.[Abstract]


Helicteres isora linn, an ayurvedic herb, is distributed widely in forest throughout India. Almost its all parts are used traditionally. The fruits are useful in diarrhoea, dysentery, wounds, ulcers, hemorrhages and diabetes. The fruit after collection, authentication and drying, was extracted with petroleum ether, chloroform, methanol and water using soxhlet extractor. The present study was aimed to isolate the constituents and evaluate the antimicrobial properties of isolated constituents of methanolic extract of fruits of Helicteres isora linn. Isolation of alkaloids, flavonoids and phenolic compounds was done by using HPTLC technique. The invitro antimicrobial activities of methanolic extract, isolated alkaloids, flavonoids and phenolic compounds were investigated against Escherichia coli, Pseudomonas aeruginosa, Salmonella abony and Staphylococcus aureus by cup-plate diffusion method. The methanolic extract showed activity against Escherichia coli, Pseudomonas aeruginosa, Salmonella abony and Staphylococcus aureus., isolated phenolic compounds against Escherichia coli, Pseudomonas aeruginosa and Salmonella abony., isolated flavonoids against Pseudomonas aeruginosa and Staphylococcus aureus., and isolated alkaloids against Escherichia coli and Staphylococcus aureus. Minimum inhibitory concentration was determined by liquid broth method. Minimum inhibitory concentration of methanolic extract against Pseudomonas aeruginosa was found to be 10μg/ml and that against Staphylococcus aureuswas found to be 8μg/ml.

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2010

Journal Article

Dr. Subin Mary Zachariah, Dr. Leena K. Pappachen, Dr. Aneesh T. P., Alex, L., Sumith, G., John, M. Sheeba, Praseetha, M. C., and Nagalekshmi, R., “Phytochemical And Pharmacological Screening Of Sphaeranthus Indicus Linn. For Antimicrobial Activity”, International Journal of Pharmaceutical Sciences and Research, vol. 1, pp. 169-173, 2010.[Abstract]


Sphaeranthus indicus Linn (Asteraceae) is a common annual spreading herb found in rice field throughout India. Six crude extracts were prepared from the whole plant Sphaeranthus indicus using different solvents by cold maceration process. The extracts were subjected to screening to detect potential antimicrobial activity against S. aureus, B. substillus, P. Gentamycin and Nystatin as standard by cup plate agar diffusion method. The aim of our present study was to find out the antimicrobial activity of the different extracts of entire part including flower heads of Sphaeranthus indicus. The different extracts such as hexane, chloroform, ethyl acetate, ethanol, methanol and aqueous extract exhibits comparable antimicrobial activity with the standard.

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2009

Journal Article

Dr. Subin Mary Zachariah, Dr. Leena K. Pappachen, Fasmi, K. N., Jose, S., and Vimal, A., “Combinatorial Drug Design-Formulation of the Era”, International Journal of Chemical Science, vol. 7, no. 3, pp. 200-2008, 2009.[Abstract]


The field of medicinal chemistry and drug design is in a state of development and is at present undergoing major restructuring. The molecular biological revolution and the progressing mapping of the human genome have created a new biochemical and biostructural great order. These developments have provided new challenges and opportunities for drug research and drug design. The aim of this review is to know more about the newly formulated drugs with the use of computer aided designed drugs and molecular modeling. One can site the advances in the understanding in the reaction mechanism, conformational analysis, host - guest interactions, quantum mechanics and so on. The major objective of the medicinal chemist is in designing molecules interacting with the degenerating process in the diseased organisms.

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2009

Journal Article

Dr. Subin Mary Zachariah and Dr. Leena K. Pappachen, “A study of genetic engineering techniques in biotechnology based pharmaceuticals”, The Internet Journal,Nanotechnology, vol. 3, no. 1, pp. 1 - 8, 2009.

2009

Journal Article

Dr. Leena K. Pappachen, Dr. Subin Mary Zachariah, and Fasmi;, K. N., “Combinatorial Drug Design- formulation of the Era.”, Int.Jour.Chem.Sci., 2009.

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