Publication Type : Journal Article
Thematic Areas : Medical Sciences
Publisher : Journal of Experimental and Clinical Cancer Research
Source : Journal of Experimental and Clinical Cancer Research, vol. 9, pp. 155-160, 1990.
Campus : Kochi
School : School of Medicine
Year : 1990
Abstract : The successful adoptive immunotherapy of the syngeneic Friend virus-induced murine leukemia FBL-3 was mediated by a proliferative MHC-restricted, tumor-specific CTL clone in combination with recombinant human IL 2. This clone was previously shown to express the L3T4-, Lyt-1+, Lyt-2+ surface phenotype. Activation of the clone for 48 hr in vitro with irradiated tumor cells induced the expression of IL 2 receptors and markedly increased clonal proliferation in response to recombinant IL 2. Intravenous injection of 2 X 10(7) 48 hr in vitro-activated cloned cells, followed by 6 days of systemic (i.p.) administration of IL 2 resulted in the complete regression of tumors and the cure of 50% of the treated mice. IL 2 alone had no effect on tumor growth, whereas the injection of nonactivated (resting) clone plus IL 2 or activated clone without IL 2 had small but insignificant effects on tumor growth and survival. These results indicated that the in vivo effector functions of cloned T cells may be markedly enhanced by the concurrent systemic administration of recombinant IL 2 and by the induction of optimal IL 2 receptor expression on the cloned T cells at the time of cell administration.
Cite this Research Publication : Dr. Damodaran Vasudevan, Suresh, K., and Nirmala, K., “Adoptive immunotherapy using human recombinant interleukin-2 activated specific cytotoxic T lymphocytes”, Journal of Experimental and Clinical Cancer Research, vol. 9, pp. 155-160, 1990.