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In silico characterisation and functional validation of chilling tolerant divergence 1 (COLD1) gene in monocots during abiotic stress

Publication Type : Journal Article

Publisher : Funct Plant Biol .

Source : Funct Plant Biol. 2019 Jun;46(6):524-532

Url : https://pubmed.ncbi.nlm.nih.gov/30940337/

Campus : Chennai

School : School of Engineering

Department : Computer Science and Engineering

Year : 2019

Abstract : The G protein-coupled receptor is one of the major transmembrane proteins in plants. It consists of an α subunit, a β subunit and three γ subunits. Chilling tolerant divergence 1 (COLD1) includes a Golgi pH receptor (GPHR) domain, which maintains cell membrane organisation and dynamics, along with abscisic acid linked G protein-coupled receptor (ABA_GPCR) that regulates the signalling pathways during cold stress. In the present study, we performed characterisation of a homologous COLD1 from the economically important monocot species Oryza sativa L., Zea mays L., Sorghum bicolor (L.)Moench and Erianthus arundinaceus (L.) Beauv. IK 76-81, a wild relative of Saccharum. COLD1 was isolated from E. arundinaceus IK 76-81, analysed for its evolution, domain, membrane topology, followed by prediction of secondary, tertiary structures and functionally validated in all four different monocots. Gene expression studies of COLD1 revealed differential expression under heat, drought, salinity and cold stresses in selected monocots. This is the first study on regulation of native COLD1 during abiotic stress in monocots, which has opened up new leads for trait improvement strategies in this economically important crop species.

Cite this Research Publication : Anunanthini P, Manoj VM, Sarath Padmanabhan TS, Dhivya S, Narayan JA, Appunu C, Sathishkumar R. In silico characterisation and functional validation of chilling tolerant divergence 1 (COLD1) gene in monocots during abiotic stress. Funct Plant Biol. 2019 Jun;46(6):524-532. doi: 10.1071/FP18189. Erratum in: Funct Plant Biol. 2019 Jun;46(6):596. PMID: 30940337.

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