Publication

Abstract : Background: Milk decoction is a unique preparation told in Ayurveda. Eventhough proved to be effective, they are not widely prescribed and used owing to its difficulty in preparation, bulkiness and very short shelf life. Milk powders were developed and used as an alternative of raw milk in food industry but no modifications of milk decoction is available in the pharmaceutical industry till date. Objective: The objective of the study is to modify milk decoction of Tribulus terrestris into dehydrated milk powder and an invivo comparison of its efficacy on spermatogenesis and sperm parameters. Design: Phyto-chemical studies of Gokshura will be done to authenticate the raw drug and to identify its active ingredients. Ksheerapaka will be prepared according to classic references and standardized, followed by the preparation of dehydrated milk powder by four methods viz Spray drying, Vaccum drying, Drum drying and Freeze drying. The testis tissue and semen will be collected from 30 Adult Wistar male rats, allocated into six groups, vehicle control (n = 5) and test groups (n = 25), of 5 that received milk decoction and milk powders prepared by different methods for 20 consequent days. Outcomes: The changes occurring in each step of preparation of decoction, milk decoction and dehydrated milk powder will be studied using LC-MS. The milk powders will be compared analytically and studied for standards laid down by FSSAI. Weight of individual male testis, sperm count, viability, motility, Serum FSH, LH, Total testosterone hormone and Histopathological study will be compared.