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Publication Type : Journal Article
Thematic Areas : Biotech
Publisher : Mikrochim Acta
Source : Mikrochim Acta, Volume 186, Issue 3, p.157 (2019)
Keywords : Bacteria, Carbon, Epithelial Cells, Fluorescent Dyes, Humans, molecular imaging, Mouth Mucosa, nitrogen, Quantum dots, Spectrometry, Fluorescence, sulfur
Campus : Amritapuri, Coimbatore
School : School of Biotechnology, School of Engineering
Center : Biosensor Research Lab, Biotechnology
Department : biotechnology, Sciences
Year : 2019
Abstract : Carbon dots co-doped with nitrogen and sulfur (NSCDs) were obtained from thiourea and TAE (Tris-acetate-ethylenediamine) buffer using microwave assisted hydrothermal synthesis. The synergistic presence of nitrogen and sulfur as a dopant results in teasing fluorescence properties and a fluorescence quantum yield of 57%. An HR-TEM study showed the NSCDs to be mono-dispersed and seemingly spherical with an average hydrodynamic diameter of 3.6 ± 0.88 nm. The NSCDs are nontoxic as proven by an MTT assay for cytotoxicity. The optical characterization was done by using UV-Vis absorption and fluorescence spectroscopy which revealed excitation wavelength-dependent multicolor emissions. The characterization of surface topology was done by using X-ray diffraction, FTIR, and X-ray photoelectron spectroscopy. The NSCDs were used to image various pathogenic bacteria (E. coli, Klebsiella, Pseudomonas & Staphylococcus) and human buccal epithelial cells by applying multicolor fluorometry. Graphical abstract Schematic presentation of microwave-assisted hydrothermal synthesis of nitrogen and sulfur doped carbon dots (NSCD) based on Thiourea and 50X Tris-acetate-ethylenediamine (TAE) buffer having multicolor fluorescence, used for tagging and imaging pathogenic bacteria and Human buccal epithelial cells using fluorescence microscope.
Cite this Research Publication : A. Pathak, Dr. Suneesh P. V., Stanley, J., and Dr. Satheesh Babu T. G., “Multicolor Emitting N/S-doped Carbon Dots as a Fluorescent Probe for Imaging Pathogenic Bacteria and Human Buccal Epithelial Cells”, Mikrochim Acta, vol. 186, no. 3, p. 157, 2019.