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Course Detail

Course Name Microbiology and Immunology
Course Code 24MMD582
Program M. Sc. Molecular Medicine(MLM)
Semester 2
Credits 3
Campus Faridabad, Kochi

Syllabus

Unit 1

Experiment 1: Observation & Identification of Immune Cells, 

Experiment 2: Observation and identification of the structure of lymphoid organs, 

Experiment 3: Blood grouping, 

Experiment 4: Separation of Mononuclear cells using density gradient centrifugation, 

Experiment 5: Isolation of Monocytes from the Mononuclear Cells, 

Experiment 6: Induction of Monocytes to M1 and M2 Macrophages, 

Experiment 7: Evaluation of inflammatory and anti-inflammatory cytokines produced by M1 and M2 macrophages through ELISA.

Experiment 8: Bacterial and fungal culture medium preparation, 

Experiment 9: Pure culture technique (eg: streaking and sub culturing), 

Experiment 10: Gram staining, Fungal staining, Motility assay 

Bacterial growth curve by measuring turbidity and viable count 

Experiment 11: Antibiotic sensitivity assay; MIC and MBC determination, 

Experiment 12: Biofilm assay, MBIC and MBEC determination, 

Experiment 13: Phage titration assay

Objectives and Outcomes

Pre-requisites:  Basic understanding of biology and biochemistry and immunology

Total number of classes:30

Preamble:

Students will be familiarized with the basics as well as advanced methods in microbiology and immunology that can be used for disease diagnosis. Provide hands-on training for isolation of microbes in pure culture and antibiotic susceptibility assays. Provide the students with practical skills on lymphocyte isolation and analysis.

Course Outcomes

CO1 Provide the students the knowledge about practical skills on basic microbiology.

CO2 Students will learn about different antimicrobial activity assays.

CO3 Students will develop an understanding about components of immune system

and their function.

CO4 The students will learn about mutagenic assays, biofilm assays and blood grouping.

CO5 The students will learn about different antigen and antibody interaction assays.

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

3

1

1

1

–

–

1

1

1

–

3

CO 2

3

3

1

1

1

–

–

1

–

1

–

3

CO 3

3

1

1

1

1

–

1

3

CO 4

3

3

1

1

1

–

–

–

–

1

–

3

CO 5

3

3

1

1

1

–

–

1

1

1

–

3

Program Specific Outcomes. (PSO)

PSO 1 – Biochemical organization and cellular complexity in function

PSO 2 – Biomolecules in Medicine 

PSO 3 – Molecular basis of disease

PSO 4 – Molecular technology in diagnosis and therapy

PSO 5 – Cellular based approaches in diagnosis and therapy

PSO 6 – Microorganisms in Medicine

PSO 7 – Nanoscale entities and its significance in Medicine 

PSO 8 – Tissue architecture engineering in Medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and biological data use

–

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

C O

CO 1

3

1

2

2

–

3

–

–

1

–

CO 2

3

–

1

2

–

–

–

–

1

–

CO 3

3

1

1

2

1

–

–

–

–

–

CO 4

3

–

1

2

–

–

–

–

–

–

CO 5

3

–

3

2

2

–

–

–

–

–

Evaluation Pattern: 30+70 = 100

Internal Assessment – 30% 

Records 

Evaluation 

30% 

30%

End Semester Examination- 50% 

Practical 

Exam 

60% 

Assessment

Viva 

10%

70%

Total

100%

Text Books / References

  1. Practical Handbook of Microbiology- Lorrence H Green, Emanuel Goldman by CRC Press, 4th Edition, ISBN 9780367567637
  2. Practical Immunology-Frank C. Hay, Olwyn M.R. Westwood, Fourth Edition,Blackwell Science.

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