Course

Genome & Genomics:

Genome mapping: Physical and Genetic Map, Genome Sequencing, Next generation sequencing methods ,Genome Annotation, Functional Genomics.

Transcriptomics:

Search for transcription factor binding sites,RNA-Seq, Microarrays, Regulatory RNAs: small or large, Computational prediction of miRNA target genes, RNA Darkmatter

Proteomics:

Basic concepts , Tools of proteomics- SDS PAGE, 2D PAGE , Liquid chromatography , Mass Spectrometry (ESI and MALDI) ,Protein identification by peptide mass fingerprinting ,Applications of proteomics.

Metabolomics:

Fundamental concept, Tools of metabolomics- Capillary electrophoresis, Gas chromatography, Electrochemical detectors, Case studies.

Lipidomics:

Basic concepts and tools Case studies

Systems Biology and Bioinformatics.

Degradomics:

• Techniques and concepts
• Approaches to identify the protease and protease-substrate repertoires, or
• ‘degradomes’, on an organism-wide scale
• Uncover new roles for proteases in vivo.
• Identification of new pharmaceutical targets to treat disease (Emerging degradomic )

1. Introduction to Proteomics -Tools for the New Biology by Daniel C. Liebler, Humana Press.
2. Mass Spectrometry for Biotechnology by Gary Siuzdak, Academic Press.
3. Proteomics for Biological Discovery by Timothy Veenstra and John Yates, Wiley.
4. Metabolomics- Methods and Protocols by Wolfram Weckwerth, Humana Press.
5. Lipidomics- Technologies and Applications by Kim Ekroos, Wiley-VCH.
6. Web/Journal Resources.
7. Transcriptomics: Expression Pattern Analysis, Virendra Gomase, Somnath Tagore; VDM Publishing, 2009 – Science