Course

Unit 1

Historical Account: Discovery of DNA as genetic material, Griffith’s experiment, Hershy and Chase warring blender experiment, Chargaff’s rule

Unit 2

Macromolecular Description: Structure of DNA, RNA and Protein Basic mechanism of replication

Unit 3

Flow Of Information-Central Dogma: Basic mechanism of replication, transcription, translation

Unit 4

Regulation In Prokaryotes and Eukaryotes: Gene regulation in prokaryotes and eukaryotes, positive regulation, negative regulation, attenuation, gene regulation in lambda phage life cycle, RNA processing and post transcriptional regulation

Unit 5

Regulatory Mechanisms: Eukaryotic transcription factors, enhancers, silencers, insulators, chromatin structure and gene regulation, Translational regulation in prokaryote and eukaryotes, Post translational modification and protein stability.

LEARNING OBJECTIVES:

Introducing and strengthening the basic molecular processes that are common to all living organisms. This course will form the pillar of knowledge which in turn helps the students for better understanding of various other subjects in the field of biotechnology.

COURSE OUTCOMES:

After completing the course, students shall be able to

CO1:Recall the important discoveries that are made in the field of molecular biology.

CO2: Understand the structure and different levels of organizations of biomolecules.

CO3: Explain the molecular events in replication, transcription and translation.

CO4: Understand the regulatory mechanisms of gene expression

1. Molecular Biology of the gene, James D Watson et al, 7^TH Edition, Pearson, 2007.
2. Gene VIII, Benjamin Lewin, 8^th edition, Pearson publishers, 2003.
3. Molecular biology, David Freifielder, 2^nd edition, Barlett and Jones, 1986.