UNIT I 3 Hours
Basics of Microbiology:
- Introduction to the science of microbiology (1hr),
- Major divisions of microbial world and Relationship among them (1hr),
- Prokaryotic & eukaryotic cells (1hr) .
UNIT II 11 Hours
Bacteria:
- Study of ultra-structure and morphological classification of bacteria (1hr),
- Nutritional requirements (1hr),
- Raw materials used for culture media, different culture medias and physical parameters for its growth (1hr),
- Bacterial growth curve, isolation, and preservation methods for pure cultures (2hrs)
- Cultivation of aerobes & anaerobes (1hr),
- Quantitative measurement of bacterial growth – total & viable counting techniques (1hr),
- Identification of bacteria using staining techniques (simple, Gram’s & acid fast staining) and biochemical tests (IMViC) (2hrs).
- Artificial Intelligence applications in microbial identification (1 hr),
- Sustainable microbiological practices – Minimizing lab waste and ensuring responsible antimicrobial use, aligned with Sustainable Development Goals (SDGs) – SDG 3, 6 & 12 (1 hr).
UNIT III 10 Hours
Fungi & viruses:
- Study of morphology(2hrs)
- Classification (2hrs),
- Reproduction/replication (3hrs),
- Pharmaceutical application (1hr),
- And cultivation of Fungi and Viruses with suitable examples (2hr).
UNIT IV 4 Hours
Rickettsiae & Spirochetes:
- Definition , morphology, structural details (2 hrs),
- Classification of Rickettsiae and Spirochetes withexamples (2hrs).
UNIT V 7 Hours
Sterilization:
- Principle, procedure, merits, demerits and applications of : Physical, chemical, gaseous sterilization (2hrs),
- Radiation and mechanical method of sterilization (1hr).
- Validation /evaluation of the efficiency of sterilization methods (1hr).
- Sterility indicators (1hr).
- Sterility testing of products including solids, liquids, ophthalmic and other sterile products, according to IP, BP and USP (2hrs).
UNIT VI 4 Hours
Disinfectants:
- Classification and mode of action of disinfectants (1hr),
- Factors influencing disinfection, antiseptics and their evaluation (2hrs).
- Evaluation of disinfectants, preservative, bactericides & Bacteriostatics (1hr).
UNIT VII 14 Hours
Immunology:
- Immunity, Definition, Classification (1hr),
- General principles of natural immunity (1hr),
- Phagocytosis, Acquired immunity (active and passive) (1hr),
- Local & herd immunity (1hr),
- Antigens, chemical nature of antigens structure and formation of Antibodies (1hr),
- Antigen-Antibody reactions (2hr),
- Bacterial exotoxins and endotoxins (1hr),
- Types of vaccines (1hr),
- Significance of toxoids in active immunity (1hr),
- Immunization programme & Importance of booster dose (1hr),
- Standardisation and method of preparation of vaccines and sera (2hrs),
- Different types of viral & bacterial vaccines including its storage requirements (1hr).
UNIT VIII 6 Hours
Diagnostic tests:
- Schick’s Test, Elisa test (1hr),
- Western Blot test, Southern Blot test (1hr),
- PCR Test with examples (1hr),
- Widal test, QBC (1hr),
- Mantaux test, Peripheral smear test (1hr),
- Study of malarial parasite (1hr)
UNIT IX 3 Hours
Microbial culture sensitivity Testing:
- Principles and methods of different microbiological assay, Assessment of a new antibiotic (2hrs).
- Methods for standardization of Penicillin, Streptomycin and Vitamin B2 and B12 (1hr).
UNIT X 7 Hours
Study of infectious diseases:
- Typhoid (1hr),
- Tuberculosis (1hr),
- Malaria (1hr),
- Cholera (1hr),
- Hepatitis and Meningitis (1hr),
- HIV, Syphilis & Gonorrhea (2hrs).
UNIT XI 6 Hours
Clinical Microbiology:
- Bacteriological examination of water, milk, food and air (2 hrs),
- Collection, processing and techniques (isolation, identification and antimicrobial susceptibility testing) for bacteriological investigations of clinical samples : Blood, urine, CSF, sputum, pus, stool, tissue etc (4 hrs).