Back close
Admissions

Course Detail

Course Name Molecular Biology Lab
Course Code 24MM681
Program M. Tech. in Molecular Medicine(MLM)
Semester 1
Credits 2
Campus Kochi

Syllabus

Isolation of chromosomal DNA from Escherichia coli;Agarose gel electrophoresis, Isolation of chromosomal DNA from human blood; Isolation of plasmid DNA from Escherichia coli; Nucleic acid quantification; Polymerase chain reaction (PCR), Restriction digestion, Restriction fragment length polymorphism (RFLP), RNA isolation from Escherichia coli; cDNA synthesis, Reverse Transcriptase PCR, DNA sequencing, Real time PCR.

Objectives and Outcomes

Pre-requisites: Basic understanding of DNA chemistry and DNA biology

Course Outcome

CO1 Explain the principles of the DNA & RNA isolation methods, PCR, agarose gel electrophoresis, sequencing methods.

CO2 Can isolate DNA, RNA, plasmids.

CO3 Can perform PCR, cDNA synthesis, RT-PCR, Real-time PCR, and sequencing.

CO4 Can follow general safety routines for laboratory work in molecular biology.

CO5 Can plan experimental work based on a protocol and critically evaluate and discuss experimental results.

*Program Outcomes (PO)(As given by NBA and ABET)

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

2

2

1

3

1

–

1

3

–

–

1

CO 2

3

2

3

3

3

1

–

1

3

–

–

1

CO 3

3

2

3

3

3

1

–

1

3

–

–

1

CO 4

2

3

3

2

2

1

–

–

–

–

–

1

CO 5

3

3

1

2

3

2

–

–

–

–

–

1

Program Specific Outcomes. (PSO)

PSO 1 – Biochemical organization and cellular complexity in function

PSO 2 – Biomolecules in Medicine

PSO 3 – Molecular basis of disease

PSO 4 – Molecular technology in diagnosis and therapy

PSO 5 – Cellular based approaches in diagnosis and therapy

PSO 6 – Microorganisms in Medicine

PSO 7 – Nanoscale entities and its significance in Medicine

PSO 8 – Tissue architecture engineering in Medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and biological data use

–

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

C O

CO 1

2

–

1

3

1

2

–

–

–

1

CO 2

–

–

–

3

1

1

–

–

–

1

CO 3

–

–

–

3

1

1

–

–

–

1

CO 4

–

–

–

3

–

–

–

–

–

–

CO 5

–

–

–

3

–

–

–

–

–

–

Evaluation Pattern

Internal Assessment – 30% 

Records 

Evaluation 

30% 
   

30%

End Semester Examination- 50% 

Practical

Exam 

60% 

Assessment

Viva 

10%
   

70%

Text Books / References

  1. Joseph Sambrook, David William Russell, Molecular Cloning: A Laboratory Manual, Volume 1-2-3 CSHL Press, 2001.
  2. Terence A. Brown, Essential Molecular Biology: A Practical Approach, Volume 1 Oxford University Press, 2000.
  3. Udo Reischl; Molecular Diagnostics of infectious diseases; Humana Press.
  4. Adrian J Harwood, Methods in Molecular Biology; Protocols for gene analysis.
  5. Frederick M Ausubel, Roger Brent, Robert D Moore, J G Seidman, John A smith, Kevin Struhl; Current protocols in Molecular Biology, John Wiley and Sons, Inc (Volume 1-4).

  6. Gene Therapy Technologies, Applications and Regulations: From Laboratory to Clini. Anthony Meager 1999 John Wiley & Sons, Ltd.

DISCLAIMER: The appearance of external links on this web site does not constitute endorsement by the School of Biotechnology/Amrita Vishwa Vidyapeetham or the information, products or services contained therein. For other than authorized activities, the Amrita Vishwa Vidyapeetham does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this web site.

Admissions Apply Now