Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Biochemical and biophysical research communications, Academic Press,
Source : Biochemical and biophysical research communications, Academic Press, Volume 362, Number 3, p.619–625 (2007)
Url : http://www.sciencedirect.com/science/article/pii/S0006291X07017196
Keywords : 20β-DP; Cortisol; Transcriptional regulation; Medaka, P450c17-I and -II; E2; 17α
Campus : Kochi
School : Center for Nanosciences
Center : Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2007
Abstract : Recently we reported the isolation of two types of cytochrome P450c17s (steroid 17α-hydroxylase/C17, 20 lyase) encoded by two different genes, from genomes of teleost fish. In this study, we characterized the expression profile, enzymatic activity and transcriptional regulation of P450c17-I and -II in the medaka ovary. Similar to tilapia, medaka P450c17-I possessed both hydroxylase and lyase activities, while P450c17-II possessed only the hydroxylase activity. In situ hybridization and gene expression profiles during 48 h prior to spawning indicated that P450c17-I is responsible for the production of estradiol-17β during oocyte growth, while P450c17-II for the production of 17α, 20β-dihydroxy-4-pregnen-3-one during oocyte maturation and cortisol production in the head kidney. Luciferase assays and expression profiles of transcription factors as revealed by real time PCR suggested that P450c17-I and -II expression are tightly controlled by Ad4BP/SF-1, Lrh-1, Foxl2, and Dax1 during the 48 h prior to spawning.
Cite this Research Publication : L. - Y. Zhou, Wang, D. - S., Shibata, Y., Dr. Bindhu Paul, Suzuki, A., and Nagahama, Y., “Characterization, expression and transcriptional regulation of P450c17-I and-II in the medaka, Oryzias latipes”, Biochemical and biophysical research communications, vol. 362, pp. 619–625, 2007.